BJ-5ta (ATCC® CRL-4001)

Organism: Homo sapiens, human  /  Cell Type: Fibroblast immortalized with hTERT  /  Tissue: Foreskin  /  Disease: Normal

Permits and Restrictions

View Permits View Restrictions

Organism Homo sapiens, human
Tissue Foreskin
Cell Type Fibroblast immortalized with hTERT
Product Format frozen
Morphology Fibroblast-like
Culture Properties Adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Normal
Age Neonatal
Gender Male
Storage Conditions Liquid nitrogen vapor phase
Karyotype This is a diploid human cell line of male origin with a modal chromosome number of 46 that occurred in 90% of the cells counted. The sex chromosomes, X and Y are both karyotypically normal.

The hTERT-immortalized foreskin fibroblast cell line, BJ-5ta, was derived by transfecting the BJ foreskin fibroblast cell line with the pGRN145 hTERT-expressing plasmid (ATCC MBA-141) at population doubling 58. Cells were cultured in medium containing hygromycin B until stable clones were selected [Pubmed: 9454332]. 

Antigen Expression

Antigen expression: Positive for fibroblast surface protein; Homo sapiens, expressed (fibroblast surface protein (FSP) was assayed by flow cytometry.).

Negative for the pan-cytokeratin epithelial marker; Homo sapiens (cytokeratins were assayed by immunocytochemistry using a pan-cytokeratin antibody).

Complete Growth Medium A 4:1 mixture of Dulbecco's medium and Medium 199 with supplements as follows :
4 parts of Dulbecco's Modified Eagle's Medium containing 4 mM L-glutamine, 4.5 g/L glucose and 1.5 g/L sodium bicarbonate
1 part of Medium 199
Supplemented with:
0.01 mg/ml hygromycin B
10% fetal bovine serum
Volumes are given for a 75 cm2 flask. Recommended use of Corning® T-75 flasks (catalog #430641). Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Add 3.0 to 5.0 mL of 0.25% trypsin-0.53 mM EDTA solution to the flask and observe cells under an inverted microscope until the cell layer is dispersed (usually within 5 to 15 minutes).
  3. Note: To avoid clumping do not hit or shake the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 10.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 3 x 103 to 5 x 103 viable cells/cm2 is recommended. Maintain cultures at a cell concentration between 8 x 103 and 1 x 104 cells/cm2.
  6. Subcultivation ratio: 1:2 to 1:3 twice weekly
  7. Incubate cultures at 37°C.
Subcultivation Ratio: 1:2 to 1:3 twice weekly
Medium Renewal: every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.
Freeze medium: culture medium, 30%; fetal bovine serum, 60%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Growth Conditions: Subculture when cell concentration reaches between 8 X 103 and 1 X 104 cells/cm2.
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8,9
D16S539: 9,13
D5S818: 12
D7S820: 11,12
THO1: 7,8
TPOX: 10,11
vWA: 16,18
Population Doubling Level (PDL) As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation.
Name of Depositor Geron Corporation
Deposited As Homo sapiens

Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332

Jiang XR, et al. Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype. Nat. Genet. 21: 111-114, 1999. PubMed: 9916802

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
  1. Number of population doublings (PDL) for ATCC® CRL-4001

    Date Updated: 3/27/2014


For commercial accounts, this cell line is only distributed under the terms of a fully signed and executed ATCC® Material Transfer Agreement and Addendum. If the commercial account is screening per completed Addendum, the recipient will be required to pay a Screening Fee (ATCC® ACS-2103™) of $4000.00.

Screening Use is defined as use of Biological Material in small molecule and biologic drug discovery, including initial target identification and validation, assay development, high throughput screening, hit identification, lead optimization, and selection of candidates for clinical development.

If the commercial account is not screening per the completed Addendum, the recipient will not be required to pay a Screening Fee.

In addition to the foregoing, this product's use is governed by the following Limited Use License. 

      1. This product may not be transferred to any third parties.
      2. This product may be used solely for internal scientific research (the “Field of Use”).  The Field of Use expressly excludes the use of the product:
          1. in a product for therapeutic or diagnostic use;
          2. in human clinical research;
          3. in the manufacture of a product intended for sale or of a component or intermediate of such a product; or
          4. in the discovery, research, development, manufacture or sale of a product that:
            1. acts by detecting or measuring telomere length, telomerase activity, telomerase RNA, or TERT mRNA or protein;
            2. acts through a response to the presence or absence of: (1) telomerase RNA, the gene encoding telomerase RNA or its promoter sequence; (2) the telomerase catalytic protein, the gene or mRNA encoding the telomerase catalytic protein, or its promoter sequence.  This includes, without limitation, a product that triggers an immune response to telomerase or any component thereof, or targets cells for a cytotoxic effect through the expression of a gene controlled by a telomerase promoter; or
            3. acts by modulating telomerase activity or telomere length (including, without limitation, inhibiting or activating telomerase).

Any use of the product outside of the Field of Use requires a separate license.  For information on obtaining a license to use this product for purposes other than those permitted in the Limited Use License, please contact ATCC at


Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332

Jiang XR, et al. Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype. Nat. Genet. 21: 111-114, 1999. PubMed: 9916802