Hank's Balanced Salt Solution (HBSS), 1X (ATCC® 30-2213)

Formulation Formulation
Product Format 500 mL
Storage Conditions Store Hank's BSS at ambient temperatures, between 15°C and 30°C
Applications For washing cells, for use as a diluent, or for use as an inorganic base for standard media preparation.
Comments
Contains no calcium, magnesium, or phenol red.
Biosafety Level N/A
Quality Control Specifications

Lot specific results are available in the Certificate of Analysis, which is available at www.atcc.org or by contacting ATCC technical service.

SPECIFICATION CERTIFICATE

Test

Specification

pH1

7.1 to 7.4

Osmolality2

256 to 286 mOsm/kg

Endotoxin3

≤ 0.25 EU/mL

Mycoplasma4

Tested Negative

Sterility5

Pass

*Please consult the Certificate of Analysis for lot-specific test results.

  1. Measured with a standard pH meter.  Meters are calibrated daily using standards traceable to the National Institute of Standards Technology using guidelines outlined in the current edition of USP.
  2. Measured with a standardized osmometer, i.e. freezing point depression.   Meters are calibrated daily using standards traceable to the National Institute of Standards Technology using guidelines outlined in the current edition of USP.
  3. Chromogenic LAL Assay using guidelines outlined by the manufacturer & FDA (December 1987), “Validation of the Limulus Amebocyte Lysate Test as an End Product Endotoxin Test for Human and Animal Parenteral Drugs, Biological Products, and Medical Devices”.
  4. Large volume method of Kern and Barile as per “Isolation of Mycoplasmas from Cell Culture by Agar and Broth Techniques,” M.F. Barile and G.J. McGarrity in Methods in Mycoplasmology, Vol. 11.5 Razin and J.G. Tulley, eds., Academic Press, NY (1983) pp. 159-165.
  5. Tested by membrane filtration techniques.

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