IRR-MRC-5 [irradiated MRC-5] (ATCC® 55-X™) Organism: Homo sapiens, human / Cell Type: fibroblast / Tissue: lung / General Information Characteristics Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits Organism Homo sapiens, human Tissue lung Cell Type fibroblast Product Format frozen Morphology fibroblast Culture Properties adherent Biosafety Level 1 Age 14 weeks gestation Gender male Ethnicity Caucasian Applications These cells are provided to be used as feeder cells to support the growth of other cells. Clinical Data 14 weeks gestationCaucasianmale Comments These cells have been growth-arrested by irradiation with 10,000 rads and will begin to deteriorate 2 weeks after plating. We recommend that you do not keep the cells in culture for longer than 2 weeks. Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Culture Conditions Temperature: 37°C Atmosphere: Air, 95%; carbon dioxide (CO2), 5% Name of Depositor JP Jacobs Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis SDS