Y3-Ag 1.2.3 (ATCC® CRL-1631)

Organism: Rattus norvegicus, rat  /  Cell Type: B lymphoblast  /  Disease: plasmacytoma; myeloma

Permits and Restrictions

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Organism Rattus norvegicus, rat
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease plasmacytoma; myeloma
Strain Lou
Applications
No commercial use or third party distribution is permitted without the concurrence of C. Milstein.
The cells are resistant to 8-azaguanine, are HAT sensitive and can be used as a fusion partner for rat B cells to make rat - rat hybridomas.
Tested and found negative for ectromelia virus (mousepox).
Derivation
This is a derivative of the S210 myeloma developed by C. Milstein.
Genes Expressed
immunoglobulin
Comments
This is a derivative of the S210 myeloma developed by C. Milstein.
No commercial use or third party distribution is permitted without the concurrence of C. Milstein.
The cells are resistant to 8-azaguanine, are HAT sensitive and can be used as a fusion partner for rat B cells to make rat - rat hybridomas.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype kappa light chain
Name of Depositor K Yamada
References

Galfre G, et al. Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG. Nature 277: 131-133, 1979. PubMed: 310519

Bazin H, et al. Transplantable immunoglobulin-secreting tumours in rats. I. General features of LOU-Ws1 strain rat immunocytomas and their monoclonal proteins. Int. J. Cancer 10: 568-580, 1972. PubMed: 4668496

Basic Documentation
Restrictions

the depositor has provided these cells for research purposes only and with the following restrictions: (1) no distribution should be made to third parties, (2) any proposed commercial use must be negotiated with the National Research Development Corporation, 101 Newington Causeway, London SE1 6BU, (3) all papers reporting any use of these or derived clones should make direct reference to the original publication (Nature 277:131-133, 1979)

References

Galfre G, et al. Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG. Nature 277: 131-133, 1979. PubMed: 310519

Bazin H, et al. Transplantable immunoglobulin-secreting tumours in rats. I. General features of LOU-Ws1 strain rat immunocytomas and their monoclonal proteins. Int. J. Cancer 10: 568-580, 1972. PubMed: 4668496