S194/5.XXO.BU.1 (ATCC® TIB-20)

Organism: Mus musculus, mouse  /  Cell Type: B lymphocyte  /  Disease: plasmacytoma; myeloma

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Organism Mus musculus, mouse
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease plasmacytoma; myeloma
Strain BALB/c
Applications
Resistant to 0.1 mM 5-bromo-2'-deoxyuridine.
Derived from the S194/5.XXO-1 cell line (ATCC TIB-19).
Tested and found negative for ectromelia virus (mousepox).
Derivation
Derived from the S194/5.XXO-1 cell line (ATCC TIB-19).
Antigen Expression
H-2d; PC.1
Genes Expressed
immunoglobulin; monoclonal antibody; not secreted,H-2d; PC.1
Cellular Products
immunoglobulin; monoclonal antibody; not secreted
Comments
Resistant to 0.1 mM 5-bromo-2'-deoxyuridine.
Derived from the S194/5.XXO-1 cell line (ATCC TIB-19).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgA (not secreted); kappa light chain (not secreted)
Name of Depositor R Hyman, P Ralph, S Sarkar
References

Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881