C58(NT)D.1.G.OUAR.1 (ATCC® TIB-236)

Organism: Rattus norvegicus, rat  /  Cell Type: T lymphocyte  /  Disease: lymphoma

Permits and Restrictions

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Organism Rattus norvegicus, rat
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Strain WFu
Applications
The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.
The line may be useful for the production of rat - rat T cell hybridomas.
Storage Conditions liquid nitrogen vapor temperature
Comments
The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.
The line may be useful for the production of rat - rat T cell hybridomas.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor R Hyman
References

Hyman R, Trowbridge I. Two complementation classes of T200 (Ly-5) glycoprotein-negative mutants. Immunogenetics 12: 511-523, 1981. PubMed: 7194313

Geering G, et al. Antigens of leukemias induced by naturally occurring murine leukemia virus: their relation to the antigens of gross virus and other murine leukemia viruses. J. Exp. Med. 124: 753-772, 1966. PubMed: 4288480

Curling E, et al. Isolation of rat/rat T cell hybrids using W/Fu C58(NT)D as fusion partner. J. Immunol. Methods 108: 171-178, 1988. PubMed: 2965188

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Hyman R, Trowbridge I. Two complementation classes of T200 (Ly-5) glycoprotein-negative mutants. Immunogenetics 12: 511-523, 1981. PubMed: 7194313

Geering G, et al. Antigens of leukemias induced by naturally occurring murine leukemia virus: their relation to the antigens of gross virus and other murine leukemia viruses. J. Exp. Med. 124: 753-772, 1966. PubMed: 4288480

Curling E, et al. Isolation of rat/rat T cell hybrids using W/Fu C58(NT)D as fusion partner. J. Immunol. Methods 108: 171-178, 1988. PubMed: 2965188