Geobacillus sp. (ATCC® 15952)

Strain Designations: NCA 5520  /  Type Strain: no  /  Biosafety Level: 1

Deposited As Bacillus stearothermophilus Donk
Strain Designations NCA 5520
Application
Degrades penicillin G benzylpenicillin
Produces muramoylpentapeptide carboxypeptidase D-alanine carboxypeptidase
Biotechnology
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 50°C
Atmosphere: Aerobic
Name of Depositor Nat. Canners Assn.
References

Hammarstrom S, Strominger JL. Degradation of penicillin G to phenylacetylglycine by D-alanine carboxypeptidase from Bacillus stearothermophilus. Proc. Natl. Acad. Sci. USA 72: 3463-3467, 1975. PubMed: 1059132

Despreaux CW, Manning RF. The dacA gene of Bacillus stearothermophilus coding for D-alanine carboxypeptidase: cloning, structure and expression in Escherichia coli and Pichia pastoris. Gene 131: 35-41, 1993. PubMed: 8370539

Cross References

Nucleotide (GenBank) : X68587 B.stearothermophilus dacA gene for D-alanine carboxypeptidase.