Escherichia coli (Migula) Castellani and Chalmers (ATCC® 11775-MINI-PACK)

Strain Designations: NCTC 9001  /  Type Strain: yes  /  Biosafety Level: 2   [Based upon a review of this item in October 2016 the BSL level was determined to be BSL 2.]

Permits and Restrictions

View Permits

Classification Enterobacteriaceae, Escherichia
Strain Designations NCTC 9001
Application ATCC® 11775-MINI-PACK™ consists of 6 ready-to-use vials of ATCC® 11775™ frozen in 200 µL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.  Learn more about ATCC® MinisDeveloped by the leaders in microbial cultivation and preservation, ATCC® Minis provide a convenient, ready-to-use solution for handling quality control strains. ATCC® Minis are authenticated and backed by ATCC polyphasic testing – ensuring the same consistent and reliable reference materials you’ve come to trust for ATCC Genuine Cultures®. It is easy to ensure the quality of your products with ATCC® Minis – just open, plate, and go! .

Control Culture
Detection of aerosols
Media testing
Quality control strain
Reference material
Quality control strain for API products
Water testing
Isolation Urine
Biosafety Level 2   [Based upon a review of this item in October 2016 the BSL level was determined to be BSL 2.]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Antigenic Properties Serovar O1:K1:H7
Product Format frozen
6 ready-to-use vials of ATCC® 11775™ in glycerol stock
Storage Conditions -70°C or colder, store upright. Storage at -20°C is acceptable for up to 1 year.
Preceptrol® no
Type Strain yes
Antigenic Properties Serovar O1:K1:H7
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor NCTC
Special Collection ATCC® Minis
Chain of Custody ATCC <-- NCTC <-- F Kaufmann U 5/41
Isolation Urine
References

Int. Bull. Bacteriol. Nomencl. Taxon. 13: 153, 1963.

Lange JL, et al. Application of flow cytometry and fluorescent in situ hybridization for assessment of exposures to airborne bacteria. Appl. Environ. Microbiol. 63: 1557-1563, 1997. PubMed: 9097451

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

API Rapid 20E. bioMerieux.

Colilert. IDEXX.

Colisure. IDEXX.

Food Microbiology. Methods2.12: Examination for specific organisms--Escherichia coli in bivalve molluscs--Rapid method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 1766.2.12:2002.

Water microbiology. Method 6: Thermotolerant coliforms and Escherichia coli--Estimation of most probable number (MPN). Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.6-1995.

Water microbiology. Method 7: Thermotolerant coliforms and Escherichia coli -- Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.7-1995.

Water microbiology. Method 12: Pseudomonas aeruginosa--Estimation of most probable number(MPN). Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.12-1995.

Water microbiology. Method 13: Pseudomonas aeruginosa- Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.13-1995.

Water microbiology. Method 16: Bactericidal efficiency of water disinfecting tablets. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 4276.16:1999.

Water microbiology. Method 19: Examination for thermophilic Campylobacter spp. --Membrane filtration. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 4276.19:2001.

Escherichia coli in Water by Membrane Filtration using membrane-Thermotolerant Escherichia coli Agar (mTEC). Washington, DC:Environmental Protection Agency;EPA EPA Method 1103.1, 2005

Enterococci in Water by Membrane Filtration Using membrane-Enterococcus-Esculin Iron Agar. Washington, DC:Environmental Protection Agency;EPA EPA Method 1106.1, 2005

Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl-B-D-Glucoside Agar. Washington, DC:Environmental Protection Agency;EPA EPA Method 1600, 2005

Escherichia coli in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar. Washington, DC:Environmental Protection Agency;EPA EPA Method 1603, 2005

Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermenation using Lauryl Tryptose Broth (LTB) and EC Medium. Washington, DC:Environmental Protection Agency;EPA EPA Method 1680, 2005

Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.

Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.

E*Colite™ Test/ColiGel™ Test. Charm.

Colilert-18 QC Set. IDEXX.

ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)

Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.

GEN III MicroPlate™ QC/ OmniLog® & MicroStation™ Validation Set

API ID 32 E. bioMerieux.

Pathogel™ Test. Charm.

Cross References

Nucleotide (GenBank) : AF052254 Escherichia coli DNA gyrase A (gyrA) gene, partial cds.

Nucleotide (GenBank) : AX110211 Sequence 944 from Patent WO0123604.

Nucleotide (GenBank) : AX110241 Sequence 974 from Patent WO0123604.

Nucleotide (GenBank) : X80725 E.coli (ATCC 11775T) gene for 16S rRNA.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation