Quantitative Genomic DNA from Bacteroides fragilis (ATCC® 25285DQ)

Type Strain: yes  /  Biosafety Level: 1

Permits and Restrictions

View Permits

Application ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Specification range: ≥1 x 105 copies/µL
100 µL per vial
Storage Conditions -20°C or colder
Preceptrol® no
Genome Sequenced Strain

Bacteroides fragilis NCTC 9343, complete genome

Type Strain yes
Comments Genomic DNA isolated from a preparation of Bacteroides fragilis strain VPI 2553 (ATCC® 25285™). The source organism is also available through the ATCC catalog.
Name of Depositor ATCC
References

Cato EP, Johnson JL. Reinstatement of species rank for Bacteroides fragilis, B. ovatus, B. distasonis, B. thetaiotaomicron, and B. vulgatus: Designation of neotype strains for Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers and Bacteroides thetaiotaomicron (Distaso) Castellani and Chalmers. Int. J. Syst. Bacteriol. 26: 230-237, 1976.

Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M11-A7.

Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Takahata M, Nishino T. Antibacterial activities of tosufloxacin against anaerobic bacteria and the electron micrograph of its bactericidal effects. Chemotherapy 43: 153-158, 1997. PubMed: 9142454

BD Crystal ANR QC Set. BD Becton Dickinson; Crystal

MicroScan Rapid Anaerobe ID QC Set. Dade/Siemens.

Sensititre™ Anaerobe MIC Susceptibility. Thermo Scientific™ Sensititre™ Systems, Trek Diagnostic Systems.

Quality Control of Microbiological Transport Systems; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M40-A.

Medical microbiology--Culture media -- Part 4: Transport systems for specimens containing bacteria. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-4: 2003, 2003

Cross References

Nucleotide (GenBank) : X83935 B.fragilis 16S rRNA (ATCC 25285T).

Nucleotide (GenBank) : AX110196 Sequence 929 from Patent WO0123604.

Nucleotide (GenBank) : AF280705 Bacteroides fragilis isolate ATCC 25285 RecA (recA) gene, partial cds.

Nucleotide (GenBank) : U05887 Bacteroides fragilis ATCC 25285 beta-lactamase (cepA-85) gene, complete cds.

Nucleotide (GenBank) : AF280642 Bacteroides fragilis isolate ATCC 25285 glutamine synthetase (glnA) gene, partial cds.

Nucleotide (GenBank) : CR626927 Bacteroides fragilis NCTC 9343, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation