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Aspergillus niger van Tieghem (ATCC® 6275)

Strain Designations: 4247 [AM 324, CBS 131.52, CBS 769.97, DSM 1957, IFO 6341, IMI 45551, J. Friedrich A98, KCC F-0086, NRRL 334, QM 324, QM 458, Steinberg, TC 215-4247, WB 334]  /  Product Format: freeze-dried

Permits and Restrictions

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Strain Designations 4247 [AM 324, CBS 131.52, CBS 769.97, DSM 1957, IFO 6341, IMI 45551, J. Friedrich A98, KCC F-0086, NRRL 334, QM 324, QM 458, Steinberg, TC 215-4247, WB 334]
Assay of sulfur
Degrades apple distillery waste
Fungus resistance testing
Fungus resistance testing adhesives
Fungus resistance testing aircraft transmissions
Fungus resistance testing automotive components
Fungus resistance testing baking primer
Fungus resistance testing carpets
Fungus resistance testing lacquer
Fungus resistance testing leather
Fungus resistance testing packing materials
Fungus resistance testing paint
Fungus resistance testing paper and paperboard
Fungus resistance testing plastics
Fungus resistance testing sandbags
Fungus resistance testing textiles
Fungus resistance testing varnish
Produces carboxymethyl cellulase CM-celluase
Produces citric acid citrate
Produces glucosidase, beta; acid Glucosidase; glucocerebrosidase
Produces xylan endo-1,3-beta-xylosidase xylan hydrolase, xylanase
Resistant to copper
Sterility testing
Testing slimicide
Degrades starch in wastewater
Produces carboxymethyl cellulase, beta-glucosidase, and xylanase from oil palm wastes
Produces extracellular lipases
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Preceptrol® no
Trace element nutrition studies
Leaching of aluminum from red mud
Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence


D1D2 region of the 28S ribosomal RNA gene


Beta-tubulin (bTub)


Name of Depositor C Thom
Chain of Custody
ATCC <-- C Thom <-- RA Steinberg
Cross References

Nucleotide (GenBank) : GU256739 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : KU729128 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : KU897010 beta-tubulin (TUB2) gene


Kikuchi K, et al. Method of producing carbon source for citric acid fermentation. US Patent 4,040,906 dated Aug 9 1977

Pabai F, et al. Interesterification of butter fat by partially purified extracellular lipases from Pseudomonas putida, Aspergillus niger and Rhizopus oryzae. World J. Microbiol. Biotechnol. 11: 669-677, 1995.

Prasertsan P, Oi S. Production of cellulolytic enzymes from fungi and use in the saccharification of palm cake and palm fibre. World J. Microbiol. Biotechnol. 8: 536-538, 1992.

Iwahori K, et al. Substrate permeability in pellets formed by Aspergillus niger. J. Ferment. Bioeng. 79: 387-390, 1995.

Steinberg RA. Sulfur and trace-element nutrition of Aspergillus niger. J. Agric. Res. 63: 109-127, 1941.

AATCC Antifungal Activity, Assessment on Textile Materials: Mildew and Rot Resistance of Textile Materials. Research Triangle Park, NC:American Association of Textile Chemists and Colorists;AATCC Test Method 30-2004.

AATCC Antimicrobial Activity Assessment of Carpets. Research Triangle Park, NC:American Association of Textile Chemists and Colorists;AATCC Test Method 174-1998.

ASTM International Standard Test Method for Resistance to Growth of Mold on the Surface of Interior Coatings in an Environmental Chamber. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 3273-00 (Reapproved 2005).

Deutsches Institut fur Normung Testing of plastics: Influence of fungi and bacteria; visual evaluation, change in mass or physical properties. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 53739.

IEC Basic environmental testing procedures: Mould growth. Geneva (Switzerland):International Electrotechnical Commission;IEC 60068-2-10, Part 2, Test J.

U.S. Department of Defense Environmental test methods and engineering guidelines. Washington, DC:US Department of Defense;Military Standard MIL-STD-810F, 2000

Leather, methods of sampling and testing. Washington, DC:US General Services Administration;Fed. Test Method Std. 311, 1962

Machemer WE. Use of mercury in protective coatings. Dev. Ind. Microbiol. 20: 25-39, 1979.

Katta MI, Lynd JQ. Sulfur bioassay investigations with Aspergillus niger. Bot. Gaz. 126: 120-123, 1965.

Sandvik O. Identification of moulds by serologic differentiation of their proteolytic enzymes. Acta Pathol. Microbiol. Scand. Sect. B Microbiol. 71: 333-338, 1967.

Friedrich J, et al. The use of Aspergillus niger for bioconversion of apple distillery waste. Eur. J. Appl. Microbiol. Biotechnol. 17: 243-247, 1983.

Bowman FW, et al. Collaborative study of aerobic media for sterility testing by membrane filtration. J. Pharm. Sci. 60: 1087-1088, 1971. PubMed: 5000479

Fujita M, et al. Pellet formation of fungi and its application to starch wastewater treatment. Water Sci. Technol. 28: 267-274, 1993.

Vachon P, et al. Chemical and biological leaching of aluminum from red mud. Environ. Sci. Technol. 28: 26-30, 1994.

Standard Test Method for Efficacy of Slimicides for the Paper Industry-Bacterial and Fungal Slime. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1839-07.

Environmental testing. Part 2.10: Tests--Test J and guidance: Mould growth. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 60068.2.10-2003.

Fibre Optic interconnecting divices and passive components --Basic test and measurement procedures--Part 2-16: Tests --Mould growth. Geneva (Switzerland):International Electrotechnical Commission;IEC 1300-2-16, 1995

Plastics--Evaluation of the action of micro-organisms. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 846:1997.

Plastics--Assessment of the effectiveness of fungistatic compounds in plastics formulations. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 16869:2001.

Testing of textiles -- Evaluation of the action of microfungi. London, UK:British Standards Institution;British Standard BS EN 14119:2003, 2003

Standard Specification for Poly(Vinyl Chloride) (PVC) Plastic Flexible Concealed Water-Containment Membrane. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 4551-96 (Reapproved 2001).

Standard Specification for Retroreflective Sheeting for Traffic Control . West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 4956-07e1.

Standard Test Method for Determining the Resistance of Paint Films and Related Coating to Fungal Defacement by accelerated Four-Week Agar Plate Assay. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 5590-00 (Reapproved 2005).

Standard method of evaluating the resistance of wood product surfaces to mold growth. Birmingham, AL: American Wood-Preservers' Association; AWPA E24-06, 2006

Plastics - Evaluation of the action of microorganisms. London, UK:British Standards Institution;British Standard BS EN ISO 846:1997.

Paper and board intended to come into contact with foodstuffs --- Determination of the transfer of antimicrobial constituents. London, UK:British Standards Institution;British Standard BS EN 1104:2005.

Lawrence DP, et al. Interkingdom gene transfer of a hybrid NPS/PKS from bacteria to filamentous Ascomycota. PLoS One 6: e28231, 2011. PubMed: 22140558

Geiser DM, et al. The current status of species recognition and identification in Aspergillus. Stud Mycol 59: 1-10, 2007. PubMed: 18490947

Liu YJ, Hodson MC, Hall BD. Loss of the flagellum happened only once in the fungal lineage: phylogenetic structure of kingdom Fungi inferred from RNA polymerase II subunit genes BMC Evol Biol 6: 74, 2006. PubMed: 17010206

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation