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Geobacillus stearothermophilus (Donk) Nazina et al. (ATCC® 7953-MINI-PACK)

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Type Strain: no  /  Biosafety Level: 1

Permits and Restrictions

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Deposited As Bacillus stearothermophilus Donk
Application ATCC® 7953-MINI-PACK™ consists of 6 ready-to-use vials of ATCC® 7953™ frozen in 200 µL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.  Learn more about ATCC® MinisDeveloped by the leaders in microbial cultivation and preservation, ATCC® Minis provide a convenient, ready-to-use solution for handling quality control strains. ATCC® Minis are authenticated and backed by ATCC polyphasic testing – ensuring the same consistent and reliable reference materials you’ve come to trust for ATCC Genuine Cultures®. It is easy to ensure the quality of your products with ATCC® Minis – just open, plate, and go! .

Gas sterilization control
Steam sterilization control
Sterility assurance
Sterilization control
Pharmaceutical and Personal Care
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
6 ready-to-use vials of ATCC® 7953™ in glycerol stock
Storage Conditions -70°C or colder, store upright. Storage at -20°C is acceptable for up to 1 year.
Preceptrol® no
Type Strain no
This strain is recommended by ATCC for use in the tests described in military specification MIL S-36586A and U.S. Pharmacopeia, 23rd rev., <1211>, pp. 1976-1981, 1995 where only the taxon is specified.
Stated to grow at 35°C
The ATCC lab was unable to verify growth at 35°C.
Medium ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 55°C
Atmosphere: Aerobic
Name of Depositor CP Hegarty
Special Collection ATCC® Minis

Smith NR, et al. Aerobic spore forming bacteria. US Dep Agric Monogr 16: 1-148, 1952.

Arch. Biochem. Biophys. 54: 154-161, 1955.

British Pharmacopoeia Commission Methods of sterilization. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVIII, 2003

European Pharmacopoeia Commission Biological indicators of sterilization. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 5.1.2, 1997

U.S. Pharmacopeia USP Monographs: Biological Indicator for Steam Sterilization, Paper Carrier. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011

Terminal Sterilization and Sterilization Indicators. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part II.15.

Sterilization of health care products--Biological indicators--Part 3: Biological indicators for moist heat sterilization.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11138-3:1995.

Sterilization of health care products --- Biological indicators, Part 3: Biological indicators for moist heat sterilization processes. London, UK:British Standards Institution;British Standard BS EN ISO 11138-3:2006.

Sterilization of health care products --- Biological indicators, Part 5: Biological indicators for low-temperature steam and formaldehyde sterilization processes. London, UK:British Standards Institution;British Standard BS EN ISO 11138-5:2006.

Cross References

Nucleotide (GenBank) : L13418 Bacillus stearothermophilus maltose permease (malA) gene, complete cds.

Nucleotide (GenBank) : M16814 B.stearothermophilus small acid-soluble spore protein (B-type SASP) gene, complete cds.

Nucleotide (GenBank) : M86508 Bacillus stearothermophilus proton glutamate symport protein (gltP) gene, complete cds.

Nucleotide (GenBank) : M13061 B.stearothermophilus SASP-1 gene encoding a small acid-soluble spore protein, complete cds.

Nucleotide (GenBank) : AF184067 Bacillus stearothermophilus MetS (metS) gene, partial cds; transition state regulator (abrB) gene, complete cds; and yabC pseudogene, partial sequence.

Nucleotide (GenBank) : U18943 Bacillus stearothermophilus mannitol transport protein (MtlA), putative transcriptional regulator (MtlR), mannitol enzyme IIA (MtlF) and mannitol-1-phosphate dehydrogenase (MtlD) genes, complete cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation