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MDBK (NBL-1) (ATCC® CCL-22™)

Organism: Bos taurus, cow  /  Tissue: kidney  / 

Permits and Restrictions

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Organism Bos taurus, cow
Tissue kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2  [Cells contain bovine viral diarrhea virus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age adult
Gender male
Applications
This cell line is suitable as a transfection host
Storage Conditions liquid nitrogen vapor phase
Karyotype Chromosome Frequency Distribution 50 Cells: 2n = 60. The stemline chromosome number is hypodiploid with 2S component occurring at 5%., There were a total of 11-14 marker chromosomes (4-5 metacentric, 3-4 submetacentric and 4-5 acro-telocentric) common to most hypodiploid metaphases. The X was monosomic. Neither HSR chromosomes nor DM's were seen.
Derivation
The MDBK cell line was derived from a kidney of an apparently normal adult steer, February 18, 1957, by S.H. Madin and N.B. Darby. The cells were originally obtained in July, 1967 at passage 96 from the ATCC as a frozen ampule (F-145).­
Clinical Data
male
Genes Expressed
keratin
Virus Susceptibility Vesicular stomatitis, Orsay (Indiana)
Infectious bovine rhinotracheitis
Bovine parvovirus
Bovine adenovirus 2
Bovine adenovirus 3
Bovine viral diarrhea virus 1 , Bovine viral diarrhea virus 1
Parainfluenza 3
Virus Resistance
poliovirus 2
Comments

A specific lot of CCL-22, MDBK (NBL-1) bovine kidney cells was found to be positive for bovine viral diarrhea virus (BVDV), following an investigation by ATCC (Dec. 2015). Testing for BVDV was performed in compliance with the Code of Federal Regulations, Title 9, Section 113.52 (E &F) using virus-specific fluorescent antibody (FA) technique as well as non-specific tests for hemadsorption and cytopathic effects (CPE). Samples were found to be positive for BVDV by FA. Hemadsorption and CPE were not observed in the sample inoculated cultures.  As a result,  the BSL status for CCL-22 was changed to “2”.  

Subsequent lots of CCL-22, MDBK (NBL-1) bovine kidney cells are tested for BVDV and the results will be indicated on the Certificate of Analysis for the lot.

The cells are positive for keratin by immunofluorescence
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing

Rinse the cell sheet twice with fresh 0.25% trypsin, 0.03% EDTA solution, and remove the trypsin solution. Allow the flask to stand at room temperature for 10 to 15 minutes, add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Twice per week
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor S Madin, NB Darby
Deposited As Bos taurus
Passage History

The cell line was submitted to the American Type Culture Collection by R.A. Van Deusen in passage 110 in August, 1982.

The cells were originally obtained in July, 1967 at passage 96 from the ATCC as a frozen ampule (F-145). 
Year of Origin 1957
References

Madin SH, Darby NB Jr.. Established kidney cell lines of normal adult bovine and ovine origin. Proc. Soc. Exp. Biol. Med. 98: 574-576, 1958. PubMed: 13567776

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321

Russell DW, Miller AD. Foamy virus vectors. J. Virol. 70: 217-222, 1996. PubMed: 8523528

USEPA Manual of Methods for Virology - EPA publication. Washington, DC:Environmental Protection Agency;EPA EPA 600/4-84/013 (R9), 2001

Cross References

Nucleotide (GenBank) : AF025996 Bos taurus supervillin mRNA, complete cds.

Nucleotide (GenBank) : U64997 Bos taurus ribonuclease K6 gene, partial cds.

Nucleotide (GenBank) : NC_002531 Alcelaphine herpesvirus 1, complete genome.

Nucleotide (GenBank) : AJ238405 Bos taurus mRNA for elongation factor 1 alpha.

Nucleotide (GenBank) : J05217 Bovine beta-1->4-galactosyltransferase mRNA, 5' end.

Nucleotide (GenBank) : AF005370 Alcelaphine herpesvirus 1 L-DNA, complete sequence.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
References

Madin SH, Darby NB Jr.. Established kidney cell lines of normal adult bovine and ovine origin. Proc. Soc. Exp. Biol. Med. 98: 574-576, 1958. PubMed: 13567776

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321

Russell DW, Miller AD. Foamy virus vectors. J. Virol. 70: 217-222, 1996. PubMed: 8523528

USEPA Manual of Methods for Virology - EPA publication. Washington, DC:Environmental Protection Agency;EPA EPA 600/4-84/013 (R9), 2001