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Human herpesvirus 1 (ATCC® VR-733™)

Classification: Herpesviridae, Simplexvirus  /  Product Format: 1 mL per vial

Permits and Restrictions

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Classification Herpesviridae, Simplexvirus
Deposited As Herpes simplex virus 1
Agent Human herpesvirus 1
Strain F
Common Name Herpes simplex virus 1
Applications
Susceptibility testing
Testing virucides
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 1 mL per vial
Storage Conditions -70°C or colder
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images VR-733 Images
Comments Deposited as Herpes simplex virus 1.
This strain exhibits slight cross reactivity with certain Human herpesvirus 2 monoclonals.
Effect on Host
CPE, refractile rounding and cell clumping
Recommended Host
HEp-2 (ATCC® CCL-23™)
Growth Conditions
Temperature: 34°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 34°C in a humidified 5% CO2 atmosphere while rocking continuously. End adsorption by adding virus growth medium.
Incubation: 1-3 days
Effect on Host
CPE, refractile rounding and cell clumping
Name of Depositor B Roizman, Institut Merieux
U.S. Patent
U.S. Patent Number
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Special Collection NCRR Contract
Source Facial vesicle
References

Antiviral Susceptibility Testing: Herpes Simplex Virus by Plaque Reduction Assay; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M33-A.

ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002).

ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002).

Ejercito PM, et al. Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. J. Gen. Virol. 2: 357-364, 1968. PubMed: 4300104

Frankel N, Roizman B. Herpes simplex virus: genome size and redundancy studied by renaturation kinetics. J. Virol. 8: 591-593, 1971. PubMed: 4331657

Kieff ED, et al. Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. J. Virol. 8: 125-132, 1971. PubMed: 4329966

Kieff ED, et al. Genetic relatedness of type 1 and type 2 herpes simplex viruses. J. Virol. 9: 738-745, 1972. PubMed: 4337161

Roizman B. Perspectives in Virology VIII. New York, NY: Academic Press; .

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
Other Documentation
Other Document
Micrograph of Host Effects