Certified Reference Materials (CRMs)

• Human DNA for Whole-Genome Variant Assessment (Daughter of Utah/European Ancestry) (ATCC^® NIST-8398^™)

  ATCC^® Number: NIST-8398^™
  

  Storage: NIST-8398 is stored at −20°C for long-term storage, or in the dark at 4°C for short-term storage (if use is imminent). Please limit freeze-thaw cycles

  Applications: This Reference Material (RM) is intended for assessing performance of human genome sequencing, including whole genome sequencing, whole exome sequencing, and more targeted sequencing such as gene panels. Specifically, the material can be used to obtain estimates of true positives, false positives, true negatives, and false negatives for variant calls. This genomic DNA is to be analyzed as any other processed, extracted DNA. It can be used to assess sequencing library preparation, sequencing machines, and the bioinformatics steps of mapping, alignment, and variant calling. This RM is not intended to assess subsequent bioinformatics steps such as functional or clinical interpretation. 

  This RM is intended to provide a whole human genome sample and accompanying reference values to assess performance of variant calling from genome sequencing

  For-Profit:   $608.00 Non-Profit:   $608.00

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  Comments:

  This Reference Material (RM) is intended to provide a whole human genome sample and accompanying reference values to assess performance of variant calling from genome sequencing. This RM contains human genomic deoxyribonucleic acid (DNA) extracted from a large growth of the human lymphoblastoid cell line GM12878. A unit of RM 8398 consists of a single vial containing approximately 10 μg of genomic DNA, with the peak of the nominal length distribution longer than 48.5 kb, as referenced by Lambda DNA, and the DNA is in TE buffer (10 mM TRIS, pH 8.0, 1 mM EDTA, pH 8.0). See the NIST Certificate of Analysis for directions for use and quality control specifications.  Certificate of Analysis NIST SDS

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  • SDS

• Pseudomonas aeruginosa (ATCC^® CRM-9027^™)

  ATCC^® Number: CRM-9027^™
  

  Deposited As Pseudomonas aeruginosa (Schroeter) Migula

  Strain Designations: R. Hugh 813

  Type Strain: no

  Biosafety Level: 2

  For-Profit:   $420.00 Non-Profit:   $315.00

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  Biosafety Level:	2
  Product Format:	freeze-dried

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• Mycoplasma pneumoniae Somerson et al. (ATCC^® qCRM-15531D^™)

  ATCC^® Number: qCRM-15531D^™
  

  Strain Designations: Genomic DNA from Mycoplasma pneumoniae FH strain of Eaton Agent [NCTC 10119]

  Type Strain: yes

  Biosafety Level: 1

  For-Profit:   $490.00 Non-Profit:   $490.00

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  Biosafety Level:	1
  Product Format:	frozen Specification range: 1x106 - 1x107 genome copies/µL

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• Mycoplasma orale (ATCC^® qCRM-23714D^™)

  ATCC^® Number: qCRM-23714D^™
  

  Strain Designations: Genomic DNA from Mycoplasma orale strain CH 19299 [NCTC 10112]

  Type Strain: yes

  Biosafety Level: 1

  For-Profit:   $490.00 Non-Profit:   $490.00

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  Biosafety Level:	1
  Product Format:	frozen Specification range: 1x106 - 1x107 genome copies/µL

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• High molecular weight genomic DNA isolated from PSN-1; Pancreas Adenocarcinoma; Human cell line. (ATCC^® CRM-CRL-3211D^™)

  ATCC^® Number: CRM-CRL-3211D^™
  

  Quantity: 5 μg

  Storage: -80°C or colder

  Applications: For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009.

  For-Profit:   $408.00 Non-Profit:   $408.00

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  Product Format:

  frozen 5 μg

  Comments:

  Store at - 80°C or colder in a manual defrost freezer.  Avoid freeze-thawing.  Alternatively, product can be stored at -20°C or colder, but stability at this temperature has not been tested by the ATCC and is not guaranteed. Certified Reference Material produced under an ISO Guide 34:2009 accredited process. This item has been tested for KRAS mutation: Homozygous DNA Change: c.34G>C (correlates to protein sequence p.G12R).

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• HuT 78 (ATCC^® CRM-TIB-161^™)

  ATCC^® Number: CRM-TIB-161^™
  

  Organism: Homo sapiens, human

  Cell Type: Cutaneous T Lymphocyte (Kras Crm)

  Disease Sezary Syndrome

  For-Profit:   $714.00 Non-Profit:   $535.50

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  Product Format:

  frozen

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