Certified Reference Materials (CRMs)

• Mycoplasma synoviae Olson et al. emend. Jordan et al. (ATCC^® qCRM-25204D^™)

  ATCC^® Number: qCRM-25204D^™
  

  Strain Designations: Genomic DNA from Mycoplasma synoviae strain WVU 1853 [NCTC 10124]

  Type Strain: yes

  Biosafety Level: 1

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  Biosafety Level:	1
  Product Format:	frozen Specification range: 1x106 - 1x107 genome copies/µL

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  • Certificate
    of Analysis
  • SDS

• Ca Ski (ATCC^® CRM-CRL-1550^™)

  ATCC^® Number: CRM-CRL-1550^™
  

  Organism: Homo sapiens, human

  Cell Type: Epithelial Cell (Crm)

  Tissue: Cervix; Derived Fom Metastatic Site: Small Intestine

  Disease Epidermoid Carcinoma

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  Tissue:	Cervix
  Product Format:	frozen

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• Mycoplasma pneumoniae Somerson et al. (ATCC^® qCRM-15531D^™)

  ATCC^® Number: qCRM-15531D^™
  

  Strain Designations: Genomic DNA from Mycoplasma pneumoniae FH strain of Eaton Agent [NCTC 10119]

  Type Strain: yes

  Biosafety Level: 1

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  Biosafety Level:	1
  Product Format:	frozen Specification range: 1x106 - 1x107 genome copies/µL

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• High molecular weight genomic DNA isolated from A-549 Lung Carcinoma cell line. (ATCC^® CRM-CCL-185D^™)

  ATCC^® Number: CRM-CCL-185D^™
  

  Quantity: 5 μg

  Storage: -80°C or colder

  Applications: For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009.

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  Product Format:

  5 μg

  Comments:

  Store at - 80°C or colder in a manual defrost freezer.  Avoid freeze-thawing.  Alternatively, product can be stored at -20°C or colder, but stability at this temperature has not been tested by the ATCC and is not guaranteed. Certified Reference Material produced under an ISO Guide 34:2009 accredited process. This item has been tested for KRAS mutation: Homozygous DNA Change: c.34G>A (correlates to protein sequence p.G12S).

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• A549 (ATCC^® CRM-CCL-185^™)

  ATCC^® Number: CRM-CCL-185^™
  

  Organism: Homo sapiens, human

  Cell Type: Epithelial Cell (Kras Crm)

  Tissue: Lung

  Disease Carcinoma

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  Tissue:	Lung
  Product Format:	frozen

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• SRM Genomic DNA Standards for HER2 Measurements (ATCC^® NIST-2373^™)

  ATCC^® Number: NIST-2373^™
  

  Quantity: 100 µL of DNA solution per vial

  Applications: Used to assign ratios of the human epidermal growth factor 2 gene (HER2; the official gene symbol ERBB2) to unamplified reference genes. Measurements of the amplification (increased copies) of the HER2 gene in breast cancer samples are used as a biomarker for determining the classification for breast cancers

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  Product Format:

  100 µL of DNA solution per vial

  Comments:

  SRM NIST-2373 consists of genomic DNA extracted from five breast cancer cell lines with different amounts of amplification of the HER2 gene. The five purified genomic DNAs were solubilized in a buffer consisting of 10 mmol/L tris(hydroxymethyl)aminomethane and 0.1 mmol/L ethylenediaminetetraacetic acid disodium salt (EDTA) pH 8.0 (TE–4). The five components are genomic DNA materials derived from human cell lines SK-BR-3, MDA-MB-231, MDA-MB-361, MDA-MB-453, and BT-474, labeled A, B, C, D, and E, respectively. A unit of SRM 2373 consists of five vials, one of each component, containing approximately 100 μL of DNA solution. Each of these vials is labeled and is sealed with a color-coded screw cap. See the NIST Certificate of Analysis for directions for use and quality control specifications.  Certificate of Analysis NIST SDS

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• Aspergillus brasiliensis Varga et al. (ATCC^® CRM-16404^™)

  ATCC^® Number: CRM-16404^™
  

  Deposited As Aspergillus niger van Tieghem, anamorph

  Designation: WLRI 034(120) [CBS 733.88, DSM 1387, DSM 1988, IFO 9455, IMI 149007, NCPF 2275]

  Product Format: freeze-dried

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  Comments:	Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request. Certified Reference Material produced under an ISO Guide 34:2009 accredited process. This strain was recently re-named as Aspergillus brasiliensis according to the reference Varga et al. 2007.  Use of impedance for preservative efficacy testing
  Type Strain:	no
  Biological Safety Level:	1

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