Escherichia coli (Migula) Castellani and Chalmers (ATCC® 43894)

Strain Designations: CDC EDL 932  /  Type Strain: no  /  Biosafety Level: 2

Classification Enterobacteriaceae, Escherichia
Strain Designations CDC EDL 932
Application
Media testing
Enteric research
Produces Shiga-like toxin I, Shiga-like toxin II, extracellular polysaccharide exopolysaccharide, and polysaccharide (extracellular)
Isolation
Human feces from outbreak of hemorrhagic colitis, Michigan
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Antigenic Properties Serotype O157:H7
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Antigenic Properties Serotype O157:H7
Medium ATCC® Medium 18: Trypticase Soy Agar/Broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor CDC
Isolation
Human feces from outbreak of hemorrhagic colitis, Michigan
References

Junkins AD, Doyle MP. Demonstration of exopolysaccharide production by enterohemorrhagic Escherichia coli. Curr. Microbiol. 25: 9-17, 1992. PubMed: 1369498

Kudva IT, et al. Escherichia coli O157:H7 in microbial flora of sheep. J. Clin. Microbiol. 34: 431-433, 1996. PubMed: 8789031

Marques LR, et al. Production of Shiga-like toxin by Escherichia coli. J. Infect. Dis. 154: 338-341, 1986. PubMed: 3522760

Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003.

Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.

Wells JG, et al. Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. J. Clin. Microbiol. 18: 512-520, 1983. PubMed: 6355145