Transfection reagents introduce nucleic acid constructs into eukaryotic cells for protein synthesis, overexpression of mutant DNA constructs, and RNA-targeted knockdown of protein expression.
ATCC® Transfection reagents may be used for the transfer of nucleic acids for a variety of applications, including protein expression, overexpression of mutant genes, and siRNA-targeted protein knockdown. Our transfection reagents are cationic lipid-based; due to their positive charge, these transfection reagents interact with the negatively charged backbone of nucleic acids and cell membranes. This effect, coupled with the hydrophobic nature of the associated lipids, allows the cationic lipid-DNA complex to be transported into eukaryotic cells. ATCC offers:
- TransfeX™ for adherent cultures
- GeneXPlus for suspension cultures
- New! siFEX™ siRNA- and miRNA-mediated translational knockdown
- Tailored transfection protocols for hard-to-transfect cells
- Sera and media formulated to promote recovery
- Cell lines selected for high transfectability
These exceptional transfection reagents, combined with ATCC's technical know-how, will enable your transcriptional and translational gene studies.
Select the transfection reagent for your model system below.
Transfecting suspension cultures
GeneXPlus Transfection Reagent is designed to efficiently transfect a broad spectrum of cell types. GeneXPlus displays high transfection efficiency with low cytotoxicity in suspension cells such as HEK-293T/SF, THP-1, as well as difficult-to-transfect cell lines such as RAW 264.7 and SH-SY5Y.
GeneXPlus Transfection Reagent
transfecting adherent cultures
TransfeX™ Transfection Reagent has been optimized for use on a wide range of cell types, including cells that are generally difficult to transfect, such as hTERT immortalized cell lines, primary cells, and stem cells. TransfeX™ has been optimized for transfection efficiency, low cytotoxicity, reliability, and scalability.
TransfeX™ Transfection Reagent
siFEX™ Transfection Reagent has been added to the ATCC arsenal of transfection reagents to mediate transfer of siRNA duplexes into cells for efficient gene silencing. This product has been tested in numerous cell lines, and like other ATCC reagents is optimized to exhibit low cytotoxicity as well as high transfection efficiency. siFEX™ is also amenable to scaling up, and is free of all contaminants.
siFEX™ Transfection Reagent