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Clostridium perfringens (Veillon and Zuber) Hauduroy et al.

13124-MINI-PACK

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ATCC® 13124-MINI-PACK™ consists of 6 ready-to-use vials of ATCC® 13124™ frozen in 200 µL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.
Product category
Bacteria
Strain designation
NCTC 8237 [ATCC 19408, CIP 103 409, CN 1491, NCIB 6125, NCTC 6125, S 107]
Type strain
Yes
Genome sequenced strain
Yes
Applications
Agricultural research
Bioinformatics
Media testing
Quality control
Water testing
Product format
Frozen
Shipping information
6 ready-to-use vials containing the strain in glycerol stock
Storage conditions
-80°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
ATCC® 13124-MINI-PACK™ consists of 6 ready-to-use vials of ATCC® 13124™ frozen in 200 µL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.  Developed by the leaders in microbial cultivation and preservation, ATCC® Minis provide a convenient, ready-to-use solution for handling quality control strains. ATCC® Minis are authenticated and backed by ATCC polyphasic testing – ensuring the same consistent and reliable reference materials you’ve come to trust for ATCC Genuine Cultures®. It is easy to ensure the quality of your products with ATCC® Minis – just open, plate, and go!

Media testing
Produces hemolysins
Produces lethal toxins
Produces phospholipase C lecithinase C
Quality control strain
Reference material
Control strain for anaerobe identification
Quality control strain for API, BBL, and MicroScan [Reg TM] products
Water testing
Preceptrol
No

Characteristics

Toxigenic
Yes
Toxin genes
cpa (Alpha toxin) positive
etx (Epsilon toxin) negative
pfoA (Perfringolysin O) positive
Comments
Penicillin-binding protein profile
Type A, alpha-toxigenic
The presence of cpa and pfoA genes was confirmed by PCR.
Genome sequenced strain
This strain does not produce epsilon toxin

Handling information

Medium
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
Frozen mini-cryovials packed in dry ice should either be thawed immediately for use or stored at or below
-70°C until the expiration date printed on the label. Storage at -20°C may affect the growth and viability of the culture.
  1. Thaw the bacterial strain upright using gentle agitation in a 25°C to 30°C water bath. Thawing will be rapid; approximately 2-3 minutes or until all ice crystals have melted.
  2. Immediately after thawing, wipe down the mini-cryovial with 70% ethanol. Under anaerobic conditions, aseptically transfer the entire contents to a 5-6 mL tube of #2107 broth or directly inoculate an agar slant. Best practice dictates the use of pre-reduced media.
  3. Streak several Brucella agar plates to check for colony morphology and a blood agar plate for purity.
  4. Discard the empty vial. Do not refreeze any unused portion as it will result in a loss of viability.
  5. Incubate at 37°C for 24-48 hours. Incubate Brucella agar plates anaerobically for colony formation and the blood agar plate aerobically for contamination check. Within 48 hours, growth should be evident by turbidity in the broth and by colonies on the anaerobic agar surfaces.  No growth should occur on agar plates incubated aerobically.

ANAEROBIC CONDITIONS:

Anaerobic conditions for transfer may be obtained by either of the following:

  • Use of an anaerobic gas chamber, or 
  • Placement of test tubes under a gassing cannula system connected to anaerobic gas

Anaerobic conditions for incubation may be obtained by any of the following:
  • Loose screw caps on test tubes in anaerobic chamber, 
  • Loose screw caps on test tubes in an activated anaerobic gas pack jar, or 
  • Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained. 
Handling notes
Anaerobe Systems Brucella Blood Agar plates (AS-111 or AS-141) are recommended for analyzing colony morphology.
The presence of cpa and pfoA genes was confirmed by PCR.
Purified genomic DNA of this strain is available as ATCC® 13124D-5™.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Clostridium perfringens (Veillon and Zuber) Hauduroy et al.
Depositors
NCTC
Chain of custody
ATCC <-- NCTC <-- Wellcome Lab. CN 1491
Special collection
ATCC® Minis
Cross references
GenBank AX109537 Sequence 270 from Patent WO0123604.
GenBank AX110098 Sequence 831 from Patent WO0123604.
GenBank M59103 Clostridium perfringens 16S ribosomal RNA.
GenBank J02880 C.perfringens pyruvoyl-dependent histidine decarboxylase (hdc), gene, complete cds.
GenBank S51964 {16S/23S ribosomal DNA spacer region} [Clostridium perfringens, ATCC 13124, Genomic, 239 nt]
GenBank CP00246 Clostridium perfringens ATCC 13124, complete genome.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Chalkley L, Van der Westhuyzen I. Penicillin-binding proteins of Clostridia. Curr. Microbiol. 26: 109-112, 1993.

British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003

European Pharmacopoeia Commission Microbial contamination of products not required to comply with the test for sterility (tests for specified micro-organisms). Nutritive and selective properties of the media and validity of the test for specified micro-organisms. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.6.13, 1997

Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.

US General Services Administration Test kit, anaerobe identification. Washington, DC:US General Services Administration;Commercial Item Description A-A-51238, 1985

View All Curated Citations for this Product

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