pJIR418 [JIR1961]
77387 ™
The standard reverse sequencing primer is not useful in this vector due to the duplication of the 181 bp region between pUC18 sites PvuII and EcoRI.
The order of major features in this plasmid is: 5' lacZ - EcoRI/MCS/HindIII - 3' lacZ - pIP404 ori - pIP404 rep - HpaI - catP - SmaI - AvaII - ermBP - pMB ori. Location of the major features in this plasmid : lacZ (nt 7345- 310), MCS (nt 1-57), pIP404 ori (approx. nt 1190-1419), pIP404 rep gene (nt 2702-1482), catP (nt 4830-4207), ermBP (nt 5902-5165), pMB1 ori (approx. nt 6780-7000).The vector contains the following restriction sites (position in bp): EcoRI - 1; HindIII - 57; PvuI - 175; FspI - 195; NarI - 216; Tth111 - 397; SpeI - 3235; NheI - 3549; NaeI - 3653; HpaI - 4206; EcoRV - 4723; SmaI -4984; AvaI - 5059; ScaI - 5850.