Pseudomonas aeruginosa GFP (ATCC® 10145GFP)

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Type Strain: no  /  Biosafety Level: 2

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Verified By Whole-genome Sequencing
Application Fluorescence detection
Fluorescence imaging
Cell infection assays
In vivo localization
Pathogen-host interactions assays
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain no
Antibiotic Resistance Ampicillin resistance gene (bla) encoded on a plasmid. Confers resistance to 300 µg/mL ampicillin.
Comments This clone was derived from ATCC® 10145™ and contains a multicopy vector encoding the green fluorescent protein GFPmut3. This gene is expressed under the control of the Plac promoter. This construct has been designed for Gram-negative bacteria fluorescence labeling.

Vector: pUCP18-MCSgfpmut3
Vector size: 5.492kb
Vector type: plasmid
Marker: bla (ampR)
GFPmut3: fluorescent maker under the control of Plac promoter
Origin of replication: ori from pRO1600
Unique restriction sites: BamHI, EcoRI, EcoRV, SmaI, XhoI
Double restriction sites: HindIII, PstI, SalI, XbaI
Medium ATCC® Medium 2854: Nutrient Agar/Broth with 300 mcg/ml Ampicillin
Growth Conditions Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor M Barbier
Chain of Custody Modified by ATCC, Dr. M. Barbier <-- ATCC <-- F Kavanagh <-- Merck Sharp & Dohme

Barbier M and Mittar D. Novel fluorescent reporters for studying pathogen-host interactions. ASM general meeting 2014 Poster.

Choi KH, Schweizer HP. mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat. Protoc. 1(1): 153-161, 2006. PubMed: 17406227

Opinion 36. Designation of strain ATCC 10145 as the neotype strain of Pseudomonas aeruginosa (Schroeter) Migula. Int. J. Syst. Bacteriol. 20: 15-16, 1970.

Hugh R, Leifson E. The proposed Neotype Strains of Pseudomonas aeruginosa (Schroeter 1872) Migula 1900. Int. Bull. Bacteriol. Nomencl. Taxon 14: 69-84, 1964.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
  1. Accessing genome sequencing data

    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes

    Date Updated: 9/30/2019