Thermanaerovibrio acidaminovorans (Guangsheng et al.) Baena et al.

1. Open vial according to enclosed instructions.
2. Under anaerobic conditions, withdraw 0.5 ml of the #1834 broth from a single tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube and inoculate a secondary broth with 0.1 ml of the cell suspension.  Streak #1834 agar colony morphology and streak several sheep blood agar plates to check for purity.
4. Incubate tubes, #1834 agar, and one sheep blood plate under anaerobic conditions at 55°C.  Incubate second sheep blood agar plate aerobically at 37°C for aerobic contamination check.
5. Within 72 hours, growth in broth is evident by good turbidity. 

ANAEROBIC CONDITIONS:

• Tubes of media are placed under a gassing cannula system connected to a source of oxygen-free gas.
• All transfers are performed while the test tubes are on the cannula system with a gentle stream of oxygen free gas flowing through the system.
• As the test tubes are removed from the cannula system each is sealed with butyl rubber stopper thus maintaining the anaerobic headspace.  

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