ATCC ATCC Logo 0
  • Quick Order
  • Careers
  • Support

pRS303

77138

Clone type
Vector
Applications
Molecular biology
Product format
Frozen
Buy Now
Price: $620.00 EA
Discounts may be available for our fellow nonprofit organizations. Login to see your price.

Generally ships within 1-3 business days

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
YI-type (integrating) shuttle vector
shuttle vector
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): HindIII--3.2, 1.1, 0.24; PvuII--4.0, 0.48; XbaI--4.5; EcoRI--4.5.
One of a series of pBluescript-based integrating vectors (ATCC 77138-77141) differing in the yeast selectable marker gene.
YI-type integrating shuttle vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide.
pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+).
A fragment (1.184 kb) containing the HIS3 gene was inserted into the NdeI site of pRSS56. All ends were blunted.
The following restriction sites in the multiple cloning site are no longer unique: KpnI HindIII PstI BstXI.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
4.453000068664551
Intact vector size
4.453
Vector name
pRS303 (phagemid)
Type of vector
phagemid
Construction
pRSS56 [pBluescript KS+, pBS(+)]
Host range
Saccharomyces cerevisiae
Candida robusta
Escherichia coli
Cloning sites
ApaI; XhoI; SalI; ClaI; EcoRV; EcoRI; SmaI; BamHI; SpeI; XbaI; EagI; NotI; SacII; SacI; NaeI
Insert detection
lacZ', <-
Markers
HIS3; ampR
MCS

promoter: T3, <-
replicon: pMB1
marker(s): ampR, <-
Vector: pRS303 (phagemid)
Promoters: Promoter T7
Construction: pRSS56 [pBluescript KS+, pBS(+)]
Polylinker sites
KpnI; ApaI; XhoI; ClaI; HindIII; EcoRV; EcoRI; PstI; SmaI; BamHI; SpeI; XbaI; EagI; NotI; BstXI; SacII; SacI
Promoters
T7; T3
Replicon
f1

Handling information

Host
Escherichia coli HB101 (ATCC 33694)
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): HindIII--3.2,
1.1, 0.24; PvuII--4.0, 0.48; XbaI--4.5; EcoRI--4.5.
- ATCC staff

One of a series of pBluescript-based integrating vectors (ATCC 77138-77141)
differing in the yeast selectable marker gene.
- Genetics 122: 19-27, 1989

YI-type integrating shuttle vector permitting visual detection of recombinants
and production of ssDNA in E. coli. Contains promoters for in vitro RNA
synthesis, priming sites useful for sequencing, and encodes the lacZ alpha
(lacZ') peptide.
- Genetics 122: 19-27, 1989

pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+
to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript
KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+).
- Genetics 122: 19-27, 1989

A fragment (1.184 kb) containing the HIS3 gene was inserted into the NdeI site
of pRSS56. All ends were blunted.
- Genetics 122: 19-27, 1989

History

Depositors
P Hieter
Cross references
GenBank U03435

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

Need assistance with this product? Contact our Technical Support team.

Telephone

US and Puerto Rico
800-638-6597

Outside the US
+1-703-365-2700

Hours of Operation

Monday-Friday
9:00am - 5:00pm
US Eastern Time