Stem Cell Dissociation Reagent (ATCC® ACS-3010)

Permits and Restrictions

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Product Format 250 mg
Storage Conditions 2°C to 8°C

Stem Cell Dissociation Reagent is a neutral protease isolated from Bacillus polymyxa that promotes safe and efficient detachment of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) during subcultivation in cell culture. The gentle proteolytic action of the reagent does not damage the cell membrane and supports the growth of hiPSCs in an undifferentiated state. Serum does not inhibit the activity of the reagent. Stem Cell Dissociation Reagent is suitable for hESCs and hiPSCs cultured in feeder-free and feeder-dependent conditions.

Biosafety Level N/A
Quality Control Specifications

Subcultures of hiPSCs passaged with Stem Cell Dissociation Reagent maintained pluripotency as determined by expression of pluripotent markers. Detachment of stem cells from tissue culture plastic is also demonstrated.

Unit Definition: One unit releases Folin positive amino acids from casein equivalent to 1 µmole of tyrosine per minute at 37°C and pH 7.5.

A Certificate of Analysis is available upon request.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
  1. iPSC - ATCC Stem cell dissociation reagent

    Date Updated: 1/23/2014

  2. iPSC - Storing Dissociation Reagent

    Date Updated: 1/23/2014

  3. iPSC - Dissociation reagents

    Date Updated: 1/23/2014

  4. iPSC - Removing dissociation reagent

    Date Updated: 1/23/2014


Li X. et al, ROCK inhibitor improves survival of cryopreserved serum/feeder-free single human embryonic stem cells. Human Reproduction, Vol. 24 No.3 pp 580-589, 2009. PubMed: 19056770