DB (ATCC® CRL-2289)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast  /  Tissue: Ascites  /  Disease: large cell lymphoma

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Organism Homo sapiens, human
Tissue Ascites
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease large cell lymphoma
Age 45 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Images CRL-2289 Micrograph
Derivation
The DB cell line was established by Walter J. Urba and Dan L. Longo from ascites fluid from a patient with a diffuse large cell lymphoma.
Clinical Data
45 years
Caucasian
male
Antigen Expression
CD19 +; CD20 +; CD22 +; Hle-1 +; CD21 -; CD25 -
Cellular Products
immunoglobulin
Comments
The cells do not express HLA-DR, HLA-DQ, IgM , IgD or T cell rantigen receptor.
Exposure of DB cells to phorbol esters (PMA or PdBu) that activate protein kinase C results in profound growth inhibition.
They are reported to be negative for the Epstein-Barr virus genome.
The cells were cured of a prior mycoplasma contamination by a 21 day treatment with BM-cycline.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing

Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL. Maintain cultures at a cell concentration between 5 x 105 and 2 x 106 cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL.


Medium Renewal: 2 to 3 times weekly.
Cryopreservation
Freeze Medium: Complete growth medium, 90%; DMSO, 10%
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11,12
D16S539: 11
D5S818: 11
D7S820: 11
THO1: 6,8
TPOX: 8
vWA: 15,16
Isotype IgG; lambda light chain
Name of Depositor M Beckwith
References

Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723