LTR228 [LTR 228-TG, derivative of WIL-2NS (ATCC CRL-8155)] (ATCC® HB-8502™) Organism: Homo sapiens, human / Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transforme / Tissue: spleen / Disease: hereditary spherocytosis General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits and Restrictions View Permits Organism Homo sapiens, human Tissue spleen Cell Type B lymphoblast; Epstein-Barr virus (EBV) transforme Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 2 Cells Contain HERPESVIRUS Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Disease hereditary spherocytosis Age 5 years Gender male Ethnicity Caucasian Applications The cells are resistant to 6-thioguanine and can be used in fusions with human B lymphocytes to produce human hybridomas. Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. Karyotype modal number = 48; Y present Clinical Data maleCaucasian5 years Genes Expressed immunoglobulin; monoclonal antibody (small amounts) Cellular Products immunoglobulin; monoclonal antibody (small amounts) Comments The cells are resistant to 6-thioguanine and can be used in fusions with human B lymphocytes to produce human hybridomas.The cells have a high cloning efficiency in soft agar and by limiting dilution. Complete Growth Medium Iscove's modified Dulbecco's medium with 0.03% L-glutamine and 0.02 mM 6-thioguanine, 90%; fetal bovine serum, 10% Subculturing Medium Renewal: Every 2 to 3 daysCultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Cryopreservation fetal bovine serum, 95%; DMSO, 5% STR Profile Amelogenin: X,YCSF1PO: 11,12D13S317: 11D16S539: 11,12D5S818: 12,13D7S820: 9,12THO1: 8,9.3TPOX: 8,11vWA: 17,20 Isotype IgM; kappa light chain Population Doubling Time 16 hrs Name of Depositor Cetus Corp. U.S. Patent Number 4,624,921 Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. References Raubitschek AR, et al. Human lymphoblastoid cell line and hybridomas derived therefrom. US Patent 4,624,921 dated Nov 25 1986 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis SDS References Raubitschek AR, et al. Human lymphoblastoid cell line and hybridomas derived therefrom. US Patent 4,624,921 dated Nov 25 1986