Skeletal Muscle Differentiation Tool

1. Seed ATCC PCS-950-010 (in complete growth media) onto a fibronectin coated multi-well plate, 50,000 cells/cm². 
2. Incubate @ 37°C for 24hrs. 
3. Replace complete growth media with pre-warmed differentiation media after 24hrs.  
4. Leave in differentiation media for a minimum of 96 hours or until a sufficient level of differentiation has occurred. The time to achieve differentiation will require optimization and depends on various factors including cell type, age/confluence of the culture, specific lot of smooth muscle cells, and the formulation of the differentiation medium that is used. Differentiation media should be replaced every 48 hours.
   
   Note: Under some conditions, optimal differentiation may require a period of 120 hours or more.

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