Enterovirus D68, High titer
VR-1825PQ ™
- Product category
-
Viruses
- Product type
-
Animal virusPurified virus
- Strain designation
- US/KY/14-18953
- Isolation source
- Nasopharyngeal swab from patient with respiratory illness
- Geographical isolation
- United States; Kentucky
- Applications
-
Assay developmentInfectious disease researchRespiratory disease research
- Product format
- Frozen
- Storage conditions
-
-70°C or colder
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Required Products
These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.
Detailed product information
General
- Specific applications
-
Virucide testing
Respiratory research
In vitro infectivity assay
Antiviral studies
Spiking studies
ELISpot assay
Virus neutralization assay
Virus ultrastructural studies
Immunological-based assays
PCR-based assay validation
Research and development
Assay development
Vaccine development
Characteristics
- Comments
- ATCC® VR-1825PQ™ is a high-titer, live virus suspended in 50 mM Tris-HCl + 150 mM NaCl + 1 mM EDTA + dH2O. This product was prepared from ATCC® VR-1825™ via concentration through a sucrose cushion.
Handling information
- Host
- Effects
-
CPE; cell rounding; cell sloughing
- Complete medium
-
DMEM (ATCC® 30-2002™) + 2% FBS (ATCC® 30-2020™)
- Temperature
-
33°C
- Atmosphere
-
95% Air, 5% CO2
- Incubation
- 2-3 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
- Recommendation for infection
- Plate cells 16-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking continuously at 0.5 rpm. End adsorption by adding virus growth medium.
- Handling notes
- ATCC® VR-1825PQ™ is a high-titer, live virus suspended in 50 mM Tris-HCl + 150 mM NaCl + 1 mM EDTA + dH2O. This product was prepared from ATCC® VR-1825™ via concentration through a sucrose cushion.
- Key abbreviations
-
°C, Degrees CelsiusCO2, Carbon dioxideDMEM, Dulbecco's Modified Eagle's MediumEDTA, Disodium ethylene-diaminetetraacetateFBS, Fetal bovine serumMOI, Multiplicity of infectionRD, Rhabdomyosarcoma cells
Quality control specifications
- Specification range
- >107 TCID50/mL
- Volume
- 0.5 mL
History
- Depositors
- Centers for Disease Control and Prevention
- Type of isolate
- Human
- Year of origin
- 2014
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Images
Frequently Asked Questions
References
Curated Citations
Brown BA, et al. Seven Strains of Enterovirus D68 Detected in the United States during the 2014 Severe Respiratory Disease Outbreak. Genome Announc 2(6): e01201-14, 2014. PubMed: 25414503
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