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Human respiratory syncytial virus, High titer (ATCC® VR-26PQ)

Classification: Pneumoviridae, Orthopneumovirus, Human orthopneumovirus  /  Product Format: frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 TCID50/mL

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Classification Pneumoviridae, Orthopneumovirus, Human orthopneumovirus
Deposited As Respiratory syncytial virus
Agent Human respiratory syncytial virus, High titer
Strain Long
Verified By Whole-genome Sequencing
Applications Virucide testing
Respiratory research
In vitro infectivity assay
Antiviral Studies
Spiking studies
ELISpot Assay
Virus neutralization assay
Virus ultrastructural studies
Immunological-based assays
PCR-based assay validation
Research and development
Assay development
Vaccine development
Testing virucides
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 0.5 mL per vial
Specification: Quantitative PFUs and genome copy number reported on Certificate of Analysis; Infectious Titer: >107 TCID50/mL
Storage Conditions -70°C or colder
Images VR-26PQ images
Comments ATCC® VR-26PQ™ is a high-titer, live virus suspended in 50 mM Tris-HCl + 150 mM NaCl + 1 mM EDTA + dH2O. This product was prepared from ATCC® VR-26™ via concentration through a sucrose cushion.

Non-pathogenic for mouse or guinea pig. Essentially identical to Chimpanzee coryza agent. Prototype strain for subgroup A.
Effect on Host
CPE, syncytia formation, rounding, sloughing
Recommended Host
HEp-2 (ATCC® CCL-23™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-85% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01 to 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 2-12 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Effect on Host
CPE, syncytia formation, rounding, sloughing
Name of Depositor RM Chanock, National Institute of Allergy and Infectious Diseases; ATCC
Special Collection NCRR Contract
Source 17-month-old male with pneumonia, Maryland, 1956
References

ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002).

ASTM International Standard Test Method for Efficacy of Virucidal Agents Intended for Inanimate Environmental Surfaces. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1053-97 (Reapproved 2002).

Blount RE Jr, et al. Recovery of cytopathogenic agent from chimpanzees with coryza. Proc. Soc. Exp. Biol. Med. 92: 544-549, 1956.

Chanock R, et al. Recovery from infants with respiratory illness of a virus related to chimpanzee coryza agent (CCA). I. Isolation, properties and characterization. Am. J. Hyg. 66: 281-290, 1957. PubMed: 13478578

Walpita P, et al. Distinguishing between respiratory syncytial virus subgroups by protein profile analysis. J. Clin. Microbiol. 30: 1030-1032, 1992. PubMed: 1572961

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
FAQs
  1. Accessing genome sequencing data


    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes


    Date Updated: 9/30/2019