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Optimize transfection conditions
How should I optimize transfection conditions?

  • Follow the recommended transfection condition for each cell type.
  • Monitor cellular toxicity at days 1 and 2 compared to untransfected cells.
  • Reduce the amount of DNA and transfection reagent complex  added to the cells if there is a sign of cellular toxicity.
  • Add more of DNA and transfection reagent complex to increase gene expression if there is no sign of cellular toxicity.
  • Change the medium daily to reduce cellular toxicity.
Date Created10/15/2013 03:37 PM
Date Updated03/27/2014 08:39 PM

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