It is very important that the cell monolayer is not exposed to air during the adipogenic differentiation process so that the lipid vescicles do not burst. Therefore, do not exchange 100% of the adipogenic medium at one time. Also, do not tilt the plate or aspirate the medium during medium exchange. Either of these two actions will expose all or part of the cell monolayer to air.
|Date Created||02/13/2014 02:01 PM
|Date Updated||02/13/2014 02:05 PM