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Validation - HTB-14IG

How is the IDH1 mutant-U-87 Isogenic Cell Line (ATCC® HTB-14IG ™) validated?

This line has been tested in parallel with its parental cell line at the genomic, transcript and cellular levels as well as assayed for the desired phenotypic functional activity resembling the intended genotypic IDH1R132H knock-in mutation.  Elevated 2-hydroxyglutarate or (2-HG) levels in the IDH1R132H mutant isogenic line are observed, above the WT parental cell line, and hypermethylation is detected by western blotting for H3K4me3 and H3K27me3 histone markers.

Date Created10/03/2017 11:59 AM
Date Updated10/03/2017 12:00 PM

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