FAQ's

FAQ Home

Find Answers

Isogenic cell line with RAS mutation

What type of mutation does the KRAS mutant-A375 Isogenic Cell Line (ATCC® CRL-1619IG-1 ™) contain and how was this line created?  

ATCC® CRL-1619IG-1 ™ contains a heterozygous knock-in mutation in KRAS at position c.38G>A, p.G13D generated from the parental A-375 [A375] (ATCC® CRL-1619) cells.  The mutant isogenic line was generated using the CRISPR/Cas9 gene editing system followed by single cell isolation and validation.  The A375 melanoma cell line is known to also contain the BRAFV600E mutation which shows sensitivity to BRAF-specific inhibitors.  

Date Created09/28/2017 03:37 PM
Date Updated09/28/2017 03:37 PM

Most Popular Answers

  1. Huh7 cell line
  2. ATCC HUVEC lines
  3. Passage number vs. population doubling level (PDL)
  4. U-373 MG (ATCC® HTB-17)
  5. Converting TCID[50] to plaque forming units (PFU)