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Primary vs. immortalized microvascular endothelial cells
What is the difference between primary microvascular endothelial cells and immortalized microvascular endothelial cell lines?

Primary microvascular endothelial cells may grow for 15 population doublings (PD) before they become senescent and undergo growth arrest.  The hTERT-immortalized microvascular cell lines, including ATCC® CRL-4049 ™, are able to grow for more than 25 PD without changes in endothelial cell characteristics such as expression of CD31 and the ability to take up AcLDL.  Immortalized microvascular cell lines do not undergo growth arrest at latter passages.
Date Created02/13/2014 12:15 PM
Date Updated02/13/2014 12:20 PM

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