Bdellovibrio bacteriovorus Stolp and Starr

2. Using a glass vessel containing 50 ml of the recommended broth, withdraw 0.5 to 1.0 ml and rehydrate the pellet. 

3. Incubate the tube at 30°C, and incubate the glass vessel on a reciprocal shaker at the same 
temperature.

4. After 24 hours, transfer 1 mL of the culture into a fresh tube of broth.
This tube will be held in the refrigerator to be used as a host stock, if needed. 
It will also contain the Bdellovibrio, but often a more concentrated suspension of 
the host is needed for infection.

A pure culture of the host strain, ATCC 15144 Escherichia coli, is available and may 
also be used instead of trying to propagate the host as a mixed culture.

5. After several days, examine a wet mount preparation of the glass vessel culture to check for free swimming Bdellovibrio cells. They will be observed as very tiny, actively motile, curved rods.

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