Mycobacterium avium subsp. avium Chester

1. Open vial according to enclosed instructions. 
2. Using a single tube of #1395 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette.  Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
4. Use several drops of the suspension to inoculate a #90 agar slant and/or plate of #173 agar.
5. Incubate the tubes and plate at 37°C for two weeks.

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