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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
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Shattock PM. The streptococci of Group D; the serological grouping of Streptococcus boivis and observations on serologically refractory group D strains. J. Gen. Microbiol. 3: 80-92, 1949.
Jones D, Shattock PM. The location of the group antigen of group D Streptococcus. J. Gen. Microbiol. 23: 335-343, 1960. PubMed: 13790502
Schleifer KH, Kilpper-Balz R. Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov.. Int. J. Syst. Bacteriol. 34: 31-34, 1984.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
BD Crystal Gram-Positive QC Set. BD Becton Dickinson.
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