Selenomonas ruminantium subsp. ruminantium Bryant

1. Open vial according to enclosed instructions.
2. Perform all steps under anaerobic conditions. (see below)
3. Aseptically transfer 0.5 mL of the appropriate medium to the vial and rehydrate the freeze-dried pellet.  Transfer the suspension back into the tube of broth.  Inoculate a plate of non-selective medium with 0.1 of the culture.
4. Seal the test tube with a rubber stopper and incubate anaerobically at 37°C.  Incubate the plate(s) aerobically as a purity check.
5. After two or three days, growth should be evident as indicated by turbidity through out the broth. 

ANAEROBIC CONDITIONS:

• Tubes of media are placed under a gassing cannula system hooked to a source of oxygen free gas.
• All transfers are performed while the test tubes are on the cannula system with a gentle stream of oxygen-free gas flowing through the system.
• As the test tubes are removed from the cannula system, each is sealed with butyl rubber stopper, thus maintaining the anaerobic headspace.

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