Fructilactobacillus fructivorans (Charlton et al.) Zheng et al.

1. Open vial.
2. Rehydrate the entire pellet with approximately 0.5 mL of #652 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #652 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
3. Aseptically transfer 0.5 mL of the primary broth to a #652 slant to achieve biphasic growth.
4. Use several drops of the primary broth tube to inoculate a #652 plate and/or #652 agar slant.
5. Incubate at 30°C for 5-7 days in an atmosphere of 5% CO₂.

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