Shigella dysenteriae (Shiga) Castellani and Chalmers

1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
2. Rehydrate the entire pellet with approximately 0.5 mL of #18 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #18 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
3. Use several drops of the primary broth tube to inoculate a #18 plate and/or #18 agar slant.
4. Incubate at 37°C for 24 hours.

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