iPSC-derived Monocytes; DYS0100

Human iPSC-derived Monocytes enables researchers to carry out application which are done using primary CD14+ monocytes. These cells can be used for differentiating into functionally active macrophages and dendritic cells. Additionally these cells can be used for MAT assay, drug development, toxicity screening, and cancer immunology experiments. There is an availability of large number of cell vials from a single donor.

1. Check all containers for leakage or breakage.
2. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below ­-130°C, preferably in liquid nitrogen vapor, until ready for use.

1. Using the total number of viable cells, customers have to decide seeding for their experiments and applications (see Spiller, 2015).
2. Prepare the desired combinations of culture dishes with application specific media (see Spiller, 2015). Place dishes in a 37°C, 5% CO₂, humidified incubator and allow the media to pre-equilibrate to temperature and pH for 30 minutes prior to adding cells.
3. While the culture dishes equilibrate, remove one vial of iPSC-derived Monocytes (ATCC ACS-7030) from storage and thaw the cells in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 1 to 2 minutes).
4. Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All operations from this point onward should be carried out under strict aseptic conditions.
5. Add 4ml of RPMI 1640 (ATCC 30-2001) with or without 10% FBS (ATCC 30-2020) or complete growth media for specific applications – into a sterile conical tube. Using a sterile pipette, transfer cells from the cryovial to the conical tube. Centrifuge at 200-300 x g for 5 min, remove supernatant and re suspend the pellet in required medium and take an aliquot for cell counting using Vi-cell
6. Transfer cell suspension to each of the pre-equilibrated culture dishes in the required seeding density depending the application and gently rock each dishes to evenly distribute the cells.
7. Place the seeded culture flasks in the incubator at 37°C with a 5% CO₂ atmosphere. Incubate for at least 24 hours before processing the cells further.

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.