Nitrosospira lacus

1. Thaw frozen vial at room temperature and aseptically transfer vial contents to 6 mL of #929 broth contained in an Broth tube or 10 mL of #929 broth contained in an Erlenmeyer flask.
2. Incubate flask in the dark at 26°C.   Erlenmeyer flasks can be incubated in a shaker.
3. Growth should be evident in 7 to 14 days.  After growth has been established, the culture can be transferred.  
4. When transferring the culture, use 10% inoculum.

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