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Sulfoacidibacillus thermotolerans

TSD-104

Sulfoacidibacillus thermotolerans strain Y002 is an acidophilic bacterium that was isolated from the gibbon river area from an acidic enrichment culture containing ferrous iron and yeast extract at Yellowstone National Park. This strain is grown aerobically in SJH Growth Medium.
Product category
Bacteria
Product type
Extremophile
Strain designation
Y002
Type strain
Yes
Isolation source
Gibbon river area from an acidic enrichment culture containing ferrous iron and yeast extract, Yellowstone National Park
Geographical isolation
United States
Product format
Frozen
Storage conditions
-80°C or colder
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Preceptrol
No

Characteristics

Extremophile type
Acidophile
Sulfur oxidizer
Type strain description
This culture provided to the ATCC type strain depository is neither produced nor characterized by ATCC. No technical information is available on this material. Refer to depositor for technical information on this strain.
Technical information
ATCC Technical Services does not have technical information on type strain deposits that are not fully characterized. Additional information can be found in the depositor’s publication.

Handling information

Temperature
30°C
Atmosphere
Aerobic
Incubation
2-4 days
Depositor-recommended growth conditions

SJH Growth Medium until exponential phase is reached.

SJH Growth Medium
1.80 g Galactose
25 g Tryptic Soy Broth (BD 211825)
3.80 g Ferrous sulfate (anhydrous)
0.76 g Potassium tetrathionate
DI H2O to 1 L

Mix components and adjust for pH 2.0-2.5 with H2SO4. Autoclave at 121°C. 

Basal Salts Solution
12.5 g (NH4)2SO4
5.0 g MgSO4 * 7H2O
DI H2O to 1 L

Filter sterilize.

Immediately before use, add basal salts to SJH Growth Medium to final concentration of 10% Basal Salts

History

Depositors
DB Johnson, Bangor University, Bangor, United Kingdom.
Year of origin
2000
Special collection
National Park Service (NPS) Special Collection

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Disclosures

ATCC is a private, nonprofit biological resource center (BRC) and research organization that holds deposits of new type strains. ATCC tests for viability and identity upon the initial deposit of type strains. For those strains with a “TSD” designation, no further testing is performed and these strains are made available on behalf of the depositor per the requirements of the International Journal of Systematic and Evolutionary Microbiology (IJSEM), published by the Society for General Microbiology (SGM), and the International Committee on Systematics of Prokaryotes (ICSP).

ATCC may fully accessions new type strains into its general culture collection. At that time, ATCC will provide an “ATCC” designation to the strain, fully characterize the strain, and provide a Certificate of Analysis with authentication data for that specific item.

Permits & Restrictions

Material Transfer Agreement Addendum for National Park Service Products

For every order of this item, you must provide a signed Material Transfer Agreement Addendum for National Park Service Products. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item. Email the signed addendum to [email protected] with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. If you need assistance with your order, please contact our Customer Care team or your applicable distributor.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

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