Klebsiella pneumoniae subsp. pneumoniae (Schroeter) Trevisan

BAA-2470 ^™

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Klebsiella pneumoniae subsp. pneumoniae strain 1002565 is a whole-genome sequenced bacterium that was isolated from a respiratory sample. This strain is resistant to ertapenem and imipenem and was confirmed by PCR to contain the bla_NDM gene. This bacterial culture can be used in antimicrobial resistance research, drug development, infectious disease research, and respiratory disease research.

Product category: Bacteria
Product type: Drug-resistant bacterium
Strain designation: 1002565
Type strain: No
Genome sequenced strain: Yes
Isolation source: Respiratory sample
Applications: Antimicrobial resistance research

Drug development

Infectious disease research

Respiratory disease research
Product format: Freeze-dried
Storage conditions: 2°C to 8°C

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Documentation

Product sheet

Characterization data

BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

TSB with 10% Glycerol

20-2200

General

Specific applications: Multidrug testing
Respiratory research
Preceptrol: No

Characteristics

Susceptibility profile: Resistant
Ertapenem

Imipenem
Comments: New Delhi metallo-beta-lactamase (NDM-1) positive
bla_KPC negative by PCR

bla_NDM positive by PCR
Carbepenem-resistant (Imipenem and Ertapenem)

Handling information

Medium

ATCC Medium 3: Nutrient agar or nutrient broth

ATCC Medium 2901: Nutrient Medium + 25ug/ml Imipenem

Temperature

37°C

Atmosphere

Aerobic

Handling procedure

Open vial.
Using a single tube of #3 broth, withdraw approximately 0.5 - 1 mL with a pipette. Rehydrate the entire pellet.
Aseptically transfer this aliquot back into the broth tube. Mix well.
Use several drops of this suspension to inoculate a #3 agar plate or slant.
Incubate the tubes and plate at 37°C for 24 hours.
Once growth occurs, use several colonies to inoculate a plate of #2901 agar. Only colonies carrying the NDM plasmid will grow, so pick several colonies for the transfer.
Incubate the plate at 37°C for 24 hours.
Once the culture has recovered, all future growth should be on #2901 agar or broth to prevent loss of the NDM plasmid.

Handling notes

Growth is initiated on #3 media, then switched to #2901 media with imipenem to prevent plasmid loss.
bla_KPC negative by PCR.
bla_NDM positive by PCR.
Additional information on this culture is available on the ATCC^® web site at www.atcc.org.

Quality control specifications

Verification method: Whole-genome Sequencing

History

Deposited as: Klebsiella pneumoniae subsp. pneumoniae
Depositors: K Tatum and B Limbago, CDC

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Gupta N, et al. Carbapenem-resistant Enterobacteriaceae: epidemiology and prevention. Clin. Infect. Dis. 53(1): 60-67, 2011. PubMed: 21653305

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