TS2/18.1.1
HB-195 ™
- Product category
-
Animal cells
- Product type
-
Hybridoma
- Organism
-
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
- Cell type
- hybridoma: b lymphocyte
- Morphology
- lymphoblast
- Tissue
- Spleen
- Applications
-
Immunology
- Product format
- Frozen
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Detailed product information
General
- Specific applications
-
The depositor states that the cell line should not be maintained in culture continuously for greater than four months or antibody production may be lost.Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells.Tested and found negative for ectromelia virus (mousepox).
Characteristics
- Growth properties
- Suspension
- Derivation
-
Spleen cells were fused with NS-1 myeloma cells.
- Genes expressed
- immunoglobulin, monoclonal antibody, against lymphocyte function antigen 2 (LFA-2); against CD2
- Isotype
-
Mouse IgG1
- Comments
-
Animals were immunized with human cytolytic T lymphocytes.Spleen cells were fused with NS-1 myeloma cells.The antibody will inhibit HLA DR mediated T cell cytotoxicity.The depositor states that the cell line should not be maintained in culture continuously for greater than four months or antibody production may be lost.Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells.Tested and found negative for ectromelia virus (mousepox).
Handling information
- Unpacking and storage instructions
-
- Check all containers for leakage or breakage.
- Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
- Complete medium
- The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
- Temperature
-
37°C
- Atmosphere
-
95% Air, 5% CO2
- Handling procedure
-
Part A. FROZEN CELLS
Vol./Ampule: 1.0 mL.
Recommended Handling Upon Receipt: Initiate culture as soon as possible upon
receipt. Thaw by rapid agitation in 37°C water bath. See instructions on back.
Dilute ampule contents with culture medium (see batch data above). Add fresh
medium (depending on cell density) every 2-3 days.
- Subculturing procedure
-
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.Interval: Maintain cell density between 5 X 10(4) and 2 X 10(5) viable cells/ml.Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Quality control specifications
- Mycoplasma contamination
- Not detected
History
- Deposited as
- mouse (B cell); mouse (myeloma)
- Depositors
- TA Springer
- Special collection
-
Hybridoma Bank (HB)
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
References
Curated Citations
Sanchez-Madrid F, et al. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc. Natl. Acad. Sci. USA 79: 7489-7493, 1982. PubMed: 6984191
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