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Steve Budd, MBA, has extensive experience with culturing hard-to-grow cell lines and primary cells. Watch this webinar to hear this ATCC expert cover the following key points:

  • There are many cell models; choosing the correct one for your workflow will add efficiency and productivity to your work.
  • When culturing specialty cells, such as stem cells or primary cells, certain considerations regarding the choice of media and reagents must be taken.
  • Using misidentified or cross-contaminated cell lines in experiments can invalidate experimental results; therefore, authenticating cell lines should be part of your cell culture workflow.
  • Mycoplasma infection can chronically affect the well-being of cells in culture without being detected by visual observation; multiple screening methods can be used to identify contamination.
  • Tools such as proliferation assays and cryo-containers can aid the maintenance of cell health.


Dr. Elizabeth Gillies is well-versed in using CRISPR/Cas9 genome-editing technology for creating advanced cell-based models. Watch the presentation to learn how this cutting edge technology was used to enhance virus production in ATCC cell lines

  • Most viral vaccines are manufactured in large-scale tissue culture systems using historical cell lines that are approved for vaccine manufacturing.
  • ATCC used CRISPR/Cas9 gene-editing techniques to increase the viral production efficiency of some of these historical cell lines.
  • These enhanced virus-producing cell lines have the potential to significantly reduce the costs associated with generating viral vaccines and high-titer viral stocks.

In this webinar, we will spark a discussion from a highly distinguished panel from different domains of the industry. Together, we will explore the three key areas of scientific research that play a critical role in the reproducibility science, with the aim to arrive at actionable ideas that every scientist can employ today to improve reproducibility in their own research.

Balsam Shawky, MS, and Brian Shapiro, PhD, are experts in the authentication and characterization of cell lines. Join this webinar to explore how ATCC is raising credibility in science by pioneering cutting-edge solutions that support cell line verification.

Key points:

  • Cell line authentication is a critical requirement to receive funding for research, publish papers in scientific journals, and validate in vitro studies for preclinical testing
  • ATCC offers human and mouse STR profiling services for cell line authentication
  • The mouse STR profiling method was developed by NIST in collaboration with ATCC and 10 independent laboratories

Dr. Elizabeth Gillies is well-versed in using CRISPR/Cas9 genome-editing technology for creating advanced cell-based models. Watch the presentation to learn how this cutting edge technology was used to enhance virus production in ATCC cell lines

  • Most viral vaccines are manufactured in large-scale tissue culture systems using historical cell lines that are approved for vaccine manufacturing.
  • ATCC used CRISPR/Cas9 gene-editing techniques to increase the viral production efficiency of some of these historical cell lines.
  • These enhanced virus-producing cell lines have the potential to significantly reduce the costs associated with generating viral vaccines and high-titer viral stocks.

Antimicrobial Resistance II

Arm Your Lab in the Fight Against Superbugs

2/27/2020

This second part of a two-part webinar series on antimicrobial resistance, presented by ATCC Scientist Christine Fedorchuk, will provide these key takeaways:

  • Understanding the nature of the problem: explaining the biggest challenges hindering advancement such as financial deficits, implementation of surveillance or new therapies, and the speed of resistance outpacing the speed of scientific research.
  • Explaining progress: scientific and clinical advances, how organizations are contributing to the fight against AMR, and how ATCC is contributing to the fight against AMR.

In this webinar GMU Director of the Laboratory of Molecular Virology Fatah Kachanshi and ATCC Lead Biologist Heather Branscome will cover these key points:

  • Extracellular vesicles (EVs) such as exosomes are critical mediators of intercellular communication. The diverse biological cargo that is associated with these vesicles is believed to mediate the pleiotropic effects of EVs.
  • Damaging EVs contain viral non-coding RNAs and other viral proteins. These EVs can exert deleterious effects on recipient cells; further characterization of damaging EVs may serve for diagnostic purposes.
  • Reparative EVs can contribute to various biological processes ranging from normal cellular development to the repair of damaged and/or diseased tissue. Because of their broad regenerative properties, stem cell EVs are being evaluated as potential replacements for stem cell therapy.

Antimicrobial Resistance I

A Broad-spectrum Public Health Crisis

2/13/2020

This first part of a two-part webinar series on antimicrobial resistance, presented by ATCC Scientist Christine Fedorchuk, will provide these key takeaways:

  • Understanding the true burden of antimicrobial resistant infections: explaining the global prevalence and epidemiology of drug-resistant infections, breaking down morbidity and mortality of the most critical pathogens, and underlining the astronomical costs and unsustainable toll that AMR infections take.
  • Explaining how drug resistance works: demonstrating how antibiotics and other antimicrobial compounds work, the molecular mechanisms employed by AMR pathogens to evade these drugs, and how resistance continues to spread.

In this webinar ATCC expert Kevin Grady will cover these key points:

  • Toxicologists need the high biological relevance of primary cells and the proliferative capacity of cell lines for standard, predictive assays; neither model provides both characteristics.
  • upcyte® technology offers sufficient quantities of hepatocytes that exhibit primary cell physiologies such as CYP activity. Long-term culturing is possible to detect low level hepatotoxicity.
  • upcyte® Hepatocytes are fully characterized with validated performance data, and they are tested for CYP induction and inhibition.

Despite the availability of existing genome sequences in public databases, the quality, completeness, authenticity, accuracy, and traceability of genomic data is inadequate; the lack of standards for genome quality exacerbates these underlying problems. To address the underlying problems, ATCC has implemented a robust next-generation sequencing and genome assembly workflow to produce reference-quality microbial genomes. In this webinar, ATCC genomics expert Andrew Frank, MS, will cover these key points:

  • Understand the standardized process and quality control criteria required for growing, extracting, sequencing, and analyzing each reference-quality genome
  • Review the metadata provided on the ATCC Genome Portal to contextualize the data as it fits with your NGS workflow
  • Explore the features of the ATCC Genome Portal as well as the genomes available