ATCC Transfection Reagents

Powerful Tools to Enable Genetic Manipulation

4/2/2015


Transfection technology allows for transient genetic manipulation in cell cultures that is quick, reliable, and minimally toxic. Lipid-based transfection reagents are powerful tools to investigate the role of various genes in cellular physiology. Gene expression may be enhanced by delivering DNA or mRNA for specific genes or gene products into cells. In addition, gene knockdown may be achieved through transfection of small RNA constructs, which utilize the RNAi pathway to inhibit gene expression. This webinar will provide an introduction to transfection, an overview of current transfection approaches, and a brief guide to best practices in the design and optimization of transfection experiments. Critical aspects include the cell type, culture conditions, and design of nucleic constructs. We will then focus on ATCC’s highly efficient transfection reagents, which have been optimized in a broad spectrum of cell types for a range of applications, including gene expression and siRNA-mediated knockdown.

Presenters

James Clinton, Ph.D.

James Clinton, Ph.D.,
Senior Scientist, ATCC

James Clinton, Ph.D., works in new product development, with a focus on primary cells and advanced, physiologically relevant culture systems using novel technologies. Previously he worked at University of California, San Diego and the La Jolla Institute for Molecular Medicine. Dr. Clinton attended Washington State University and University of California, San Diego where he studied Neuroscience.