Validation of differentiable molecular barcodes as reference spike-in oligonucleotides for microbiome studies

6/1/2017 — 6/5/2017

Poster presented at ASM Microbe

Abstract: A shortcoming in microbiome research is the lack of reference standards to control biases introduced by differential DNA extractability, interference with amplification, library preparation, next-generation sequencing platforms, and data analysis. The aim of this research is to develop and test novel molecular barcodes for use as spike-in reference standards in the form of oligonucleotides or recombinants in microbiome studies.