Recommended Host
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SPF chicken embryonated eggs, 9-11 days old
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Growth Conditions
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Temperature: 35°C
Recommendations for Infection: Inoculate SPF chicken embryonated eggs (intra-allantoic) at 9-11 days old. Dilute virus in D-PBS prior to inoculation.
Incubation: 2 days at 35°C in a humidified, non-CO2 atmosphere.
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