Stem Cell Culture Reagents and Media

  • Growth Kit for Neural Progenitor Cell Expansion (ATCC® ACS-3003)

    ATCC® Number: ACS-3003

    Storage: -20°C
    Applications:

    The media is specially designed for expansion of iPSC-derived neural progenitor cells (NPCs).

    Complete growth media for NPCs is DMEM: F12 (ATCC® 30-2006; not included) supplemented with the Growth Kit for Neural Progenitor Cell Expansion (ATCC® ACS-3003). To make complete NPC medium add the following components of this kit to 464 mL DMEM: F12:

    5 mL L-Alanyl-L-Glutamine
    5 mL Non-Essential Amino Acids
    10 mL NPC Growth Kit Component A
    5 mL NPC Growth Kit Component B
    1 mL NPC Growth Kit Component C
    10 mL NPC Growth Kit Component D

    For-Profit:   $310.00 Non-Profit:   $310.00

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    Product Format: frozen
    Comments: 5 mL L-Alanyl-L-Glutamine
    5 mL Non-Essential Amino Acids
    10 mL NPC Growth Kit Component A
    5 mL NPC Growth Kit Component B
    1 mL NPC Growth Kit Component C
    10 mL NPC Growth Kit Component D
    Focus Area:

    Neuronal Differentiation And Drug Screening

  • For-Profit:   $699.00 Non-Profit:   $699.00

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  • Mouse ES Cell Basal Medium (ATCC® SCRR-2011)

    ATCC® Number: SCRR-2011

    Quantity: 500 mL
    Storage: Store medium at 2°C to 8°C in the dark when not in use.
    Applications: General culture conditions to maintain undifferentiated,  pluripotent mouse ES cells are now well established, and  incremental improvements, such as the development of  Mouse ES Cell Basal Medium, are key to the ongoing  success of stem cell research.

    Mouse ES Cell Basal Medium is a sterile liquid tissue culture  medium containing phenol red that has been optimized to  support the undifferentiated self-renewal cycle related to  mouse embryonic stem cell growth.

    The medium contains a stable glutamine dipeptide, providing  a long shelf-life when handled and stored properly medium may be supplemented by addition of ES-Cell  Qualified Fetal Bovine Serum (FBS) or a serum replacement  designed for mouse ES cells. This medium can support the growth of mouse ES cells on  either a mitotically inactivated layer of mouse embryonic  fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.

    For-Profit:   $25.50 Non-Profit:   $25.50

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  • ROCK Inhibitor Y27632 (ATCC® ACS-3030)

    ATCC® Number: ACS-3030

    Quantity: 10 mg
    Storage: -20°C to -80°C

    For-Profit:   $199.00 Non-Profit:   $199.00

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    Product Format: dried 10 mg
    Comments:
    Purity is > 98% as confirmed by HPLC analysis. Structure and mass are confirmed by NMR and mass spectrometry.
    Reconstituted product can be aliquoted and stored at -20°C for up to six months. Protect from light.
  • Stem Cell Freezing Media (ATCC® ACS-3020)

    ATCC® Number: ACS-3020

    Quantity: 1 x 20 mL
    Storage: 2°C to 8°C

    For-Profit:   $51.00 Non-Profit:   $51.00

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    Product Format: 1 x 20 mL
    Comments:
    Stem Cell Freezing Media is tested for pH, appearance, and sterility. Recovery, morphology and differentiation of hESCs and hiPSCs are confirmed after cryopreservation and thawing.
  • Stem Cell Dissociation Reagent (ATCC® ACS-3010)

    ATCC® Number: ACS-3010

    Quantity: 250 mg
    Storage: 2°C to 8°C

    For-Profit:   $92.00 Non-Profit:   $92.00

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    Product Format: 250 mg
    Comments:

    Stem Cell Dissociation Reagent is a neutral protease isolated from Bacillus polymyxa that promotes safe and efficient detachment of human embryonic stem cells (hESC) and human induced pluripotent stem cells (hiPSC) during subcultivation in cell culture. The gentle proteolytic action of the reagent does not damage the cell membrane and supports the growth of hiPSCs in an undifferentiated state. Serum does not inhibit the activity of the reagent. Stem Cell Dissociation Reagent is suitable for hESCs and hiPSCs cultured in feeder-free and feeder-dependent conditions.