STR Profiling / Analysis

Searchable STR Database for Human Cell Lines

As part of our continuing efforts to characterize and authenticate the cell lines in the Cell Biology collection, ATCC has developed a comprehensive database of short tandem repeat (STR) DNA profiles for all of our human cell lines.

Search STR Profile Database

Background

Short tandem repeat (STR) loci are among the most informative polymorphic markers in the human genome. STR profiles help ensure the quality and integrity of human cell lines in the scientific community. Studies have shown that a minimum of eight (8) core STR markers are required to positively identify human cell lines. Use of 8 core STR markers (loci),D5S818, D13S317, D7S820, D16S539, vWA, TH01, TPOX, CSF1PO are recommended to (1)show relatedness between cell lines, (2) uniquely identify human cells and (3) for profile comparisons. Use of 8 core loci enables a 1 in 108 discrimination rate for unrelated individuals.1

ATCC generates human STR profiles by simultaneously amplifying multiple STR loci and amelogenin (for gender determination) using the Promega PowerPlex® Systems2. Amplicons are separated by capillary electrophoresis and analyzed using GeneMapper® software from Life Technologies. Each relevant peak in the resulting electropherogram represents an allele which is alphanumerically scored and entered into the database.

The ATCC Standards Development Organization is publishing a consensus standard (ASN-0002: Authentication of Human Cell Lines: Standardization of STR Profiling) to provide the research community with a globally-accepted methodology for assessing the identity of human cell lines. The Standard also provides guidance on the interpretation of STR profiling results. This effort should facilitate the broader adoption of cell line authentication testing into cell culture laboratory operations.

After determining the STR profiles of your human cell lines, you may compare them to the human cell lines in the ATCC STR database. Recent enhancements include: