Although there is already a wealth of knowledge on the human microbiome, much of it still remains an enigma. ATCC is committed to supporting this incredible field of research by creating the cutting-edge site-specific standards needed for studies on the mycobiome, virome, or the oral, skin, gut, and vaginal microbiomes. These standards are ideal for use as controls in microbial profiling of mixed populations or for research on how microbial communities affect human health and disease.
Explore The Human Microbiome
Figure 5. Genomic DNA mock microbial communities analyzed via 16S rRNA and shotgun metagenomics sequencing methods. Genomic DNA Mixes representing the (A) oral genomic mix (ATCC® MSA-1004™), (B) skin genomic mix (ATCC® MSA-1005™), (C) gut genomix mix (ATCC® MSA-1006™), and (D) vaginal genomix mix (ATCC® MSA-1007™) were analyzed via shotgun metagenomics and 16S rRNA sequencing. Data analyses were performed on the One Codex platform.
Explore The Virome
Figure 6. Composition of genomic DNA and whole cell virome standards. (A) ATCC Virome Standards comprise genomic nucleic acids or whole cells prepared from fully sequenced, characterized, and authenticated viral strains that are selected on the basis of genomic size, DNA or RNA genome, envelope/non-envelop, and other special features. (B) The genome copy number for each virus in the virome mixture was determined by using individual digital PCR assays. The variation observed in ATCC® MSA-2008™ could be attributed to extraction efficiency.
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Explore The Mycobiome
Figure 7. Mycobiome standards can be used with both internal transcribed spacer (ITS) and shotgun metagenomic sequencing assays. The Mycobiome Genomic DNA Mix (ATCC® MSA-1010™) was analyzed by (A) ITS and (B) shotgun metagenomic assays using the Illumina platform, and data were analyzed using OneCodex platform. ITS analysis could profile all the fungi in the mix to genus level where as shotgun metagenomic assay could identify all fungi to the species level. The results also demonstrated run-to-run reproducibility when performing both sequencing assays.
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